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H9892

Sigma-Aldrich

Hydrochloric acid

1.0 N, BioReagent, suitable for cell culture

Synonym(s):

Hydrogen chloride solution

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About This Item

Empirical Formula (Hill Notation):
HCl
CAS Number:
Molecular Weight:
36.46
MDL number:
UNSPSC Code:
12352301
PubChem Substance ID:
NACRES:
NA.71

product name

Hydrochloric acid solution, 1.0 N, BioReagent, suitable for cell culture

sterility

sterile-filtered

Quality Level

product line

BioReagent

form

solution

concentration

1.0 N

technique(s)

cell culture | mammalian: suitable

anion traces

chloride (Cl-): passes test

SMILES string

Cl

InChI

1S/ClH/h1H

InChI key

VEXZGXHMUGYJMC-UHFFFAOYSA-N

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General description

Hydrochloric acid solution, a clear colorless liquid, is a solution of hydrogen chloride gas dissolved in water. It is a strong inorganic acid.

Application

Hydrochloric acid solution (HCl solution) is mainly used to adjust the pH in cell culture medium.
It can also be used:
  • In the extraction of elderberry anthocyanins by endothelial cells.
  • To convert iron(III) perchlorate to Fe(III) ions which can then form a complex with acetohydroxamic acid (FeAHA).

The hydrochloric acid solution has been used:
  • to acidify the surface water sample and to prevent interference from carbonate concentrations up to 10 mg L-1 with organic carbon detector (OCD) detection
  • to adjust the pH of complete transfection media for transfection with chitosan/pDNA nanoparticle complexes
  • to prepare artificial tear solution (ATS) to incorporate the fluorescently tagged cholesteryl ester

Biochem/physiol Actions

Hydrochloric acid (HCl) is commonly used in research and large-scale applications. Its large-scale applications include the refinement of ore to produce tin and tantalum, the cleaning of metal products, and the hydrolysis of starch and proteins in the production of food products. HCl is also used in chemical synthesis. It can also be used in hydrolyzing samples for chromatographic analysis, such as in matairesinol, in flax seed, glycopeptides, κ-carrageenan derived oligosaccharides, and syn- and anti-1,3-diols. HCl is also used as a bleaching agent to neutralize alkaline agents in the food, textile, metal, and rubber industries.

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

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Follow our procedure for the determination of a chymotrypsin activity. This enzymatic assay of alpha chymotrypsin guides you through the entire process and necessary calculations.

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