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Pepsin from porcine gastric mucosa

Name: Pepsin from porcine gastric mucosa
Brand: SIGMA

powder, slightly beige, ≥500 U/mg

Synonym(s):

Pepsin A, Pepsin from hog stomach

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About This Item

CAS Number:

9001-75-6

UNSPSC Code:

12352204

eCl@ss:

42010127

EC Number:

232-629-3

NACRES:

NA.54

MDL number:

MFCD00081840

EC Number:

3.4.23.1 (BRENDA, IUBMB)

Specific activity:

≥500 U/mg

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Properties

form

powder

Quality Segment

200

specific activity

≥500 U/mg

mol wt

35 kDa

color

slightly beige

UniProt accession no.

P00791

storage temp.

2-8°C

Gene Information

pig ... LOC396892(396892)

Description

Application

Pepsin cleavage can be used to produce F(ab′)₂ fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.

Biochem/physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Analysis Note

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.

Other Notes

Peptide synthesis, Review.

One unit corresponds to the amount of enzyme which increases the absorbance at 280 nm by 0.001 per minute at pH 2.0 and 37 °C (Hemoglobin, Catalog No. 51290, as substrate); 15,000 absorbance units as described above are equivalent to ∼1 Bergmeyer unit. One Bergmeyer unit is the amount of enzyme which hydrolyzes 1 μmole Acetyl-L-phenyl-3,5-diiodo-L-tyrosine at pH 2.0 and 37 °C.

Peptide synthesis,citationcitation Review.citation

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This Item	P7012	P7125	77151
Sigma-Aldrich 77160 Pepsin from porcine gastric mucosa	Sigma-Aldrich P7012 Pepsin from porcine gastric mucosa	Sigma-Aldrich P7125 Pepsin from porcine gastric mucosa	Sigma-Aldrich 77151 Pepsin from porcine gastric mucosa
description powder, slightly beige, ≥500 U/mg	description lyophilized powder, ≥2,500 units/mg protein (E1%/280)	description powder, ≥400 units/mg protein	description powder, slightly beige, 1200-2400 U/mg
specific activity ≥500 U/mg	specific activity ≥2,500 units/mg protein (E1%/280)	specific activity ≥400 units/mg protein	specific activity 1200-2400 U/mg
Gene Information pig ... LOC396892(396892)	Gene Information pig ... LOC396892(396892)	Gene Information pig ... LOC396892(396892)	Gene Information pig ... LOC396892(396892)
form powder	form lyophilized powder	form powder	form powder
storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C
mol wt 35 kDa	mol wt 35 kDa	mol wt 35 kDa	mol wt 35 kDa
UniProt accession no. P00791	UniProt accession no. P00791	UniProt accession no. P00791	UniProt accession no. P00791

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Pictograms

GHS08,GHS07

Signal Word

Danger

Hazard Codes

H315,H319,H334,H335

Precautionary Statements

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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