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Key Documents

MAB3262B

Sigma-Aldrich

Anti-BrdU Antibody, clone PRB-1, biotin conjugated

clone PRB-1, Chemicon®, from mouse

Synonym(s):

BrdU

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PRB-1, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable

isotype

IgG1κ

shipped in

wet ice

target post-translational modification

unmodified

Specificity

Recognizes BrdU in ssDNA, free BrdU or BrdU coupled to a protein carrier. MAB3262 also reacts with iodouridine. Does not react with thymidine.

Immunogen

Iodouridine conjugated to ovalbumin.

Application

Flow cytometry: 1:20

Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-BrdU Antibody, clone PRB-1, biotin conjugated (Mouse Monoclonal Antibody) validated in FC to detect Bromodeoxyuridine also known as BrdU.

Physical form

Purified immunoglobulin conjugated to biotin. Liquid in PBS, pH 7.4 containing 0.05% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots up to 6 months after date of receipt. Protect from prolonged exposure to light.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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