AB2253
Anti-Doublecortin Antibody
serum, from guinea pig
Synonym(s):
Anti-Doublecortin Antibody, AB2253 | Sigma-Aldrich
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
guinea pig
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse
species reactivity (predicted by homology)
human (based on 100% sequence homology)
technique(s)
immunofluorescence: suitable (Frozen tissue sections)
immunohistochemistry: suitable (Paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... DCDC1(341019)
General description
Anti-Doublecortin, Cat. No. AB2253, is a guinea pig polyclonal antibody that detects Doublecortin and is tested for use in Immunohistochemistry (Paraffin) and Immunofluorescence, and Western Blotting.
Target molecular weight ~42 kDa observed;40.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Specificity
Reactivity with other species has not been determined.
This Guinea pig polyclonal antibody detects doublecortin in murine and rat species. It targets an epitope with in 17 amino acids from the C-terminal region.
Immunogen
Epitope: C-Terminus
Recombinant Peptide.
Application
Anti-Doublecortin Antibody has been used in immunohistochemistry and immunofluorescence staining.
Biochem/physiol Actions
Doublecortin (DCX) is identified as an important marker for neurogenesis. It serves as a valuable tool for studying and comparing the process of neurogenesis across different species.
Quality
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Doublecortin in rat brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Doublecortin in rat brain tissue lysate.
Target description
~45 kDa observed; 40.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Linkage
Replaces: AB5910
Physical form
GST Depletion
GST-Depleted Guinea Pig Sera with 0.05% Sodium Azide
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Rat Brain lysate.
Rat Brain lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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