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63052

Sigma-Aldrich

Magnesium acetate solution

BioUltra, for molecular biology, ~1 M in H2O

Synonym(s):

Magnesium acetate anhydrous, Mg(OAc)2

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About This Item

Linear Formula:
(CH3COO)2Mg
CAS Number:
142-72-3
Molecular Weight:
142.39
Beilstein:
3692530
MDL number:
MFCD00003539
UNSPSC Code:
12161700
PubChem Substance ID:
329759766
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

product line

BioUltra

form

liquid

concentration

~1 M in H2O

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

refractive index

n20/D 1.358

pH

5.0-7.0 (25 °C)

cation traces

Al: ≤5 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤5 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mn: ≤5 mg/kg
Mo: ≤1 mg/kg
Na: ≤20 mg/kg
Ni: ≤2 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.04
λ: 280 nm Amax: 0.02

SMILES string

CC(=O)O[Mg]OC(C)=O

InChI

1S/2C2H4O2.Mg/c2*1-2(3)4;/h2*1H3,(H,3,4);/q;;+2/p-2

InChI key

UEGPKNKPLBYCNK-UHFFFAOYSA-L

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Application

Magnesium acetate is used in molecular biology applications such as batch in vitro transcription of RNA and the crystallization of transcription factor:DNA complexes and proteins via the sitting-drop vapor-diffusion method.
Magnesium acetate solution has been used:
  • to supplement Midkin fused to the biotin ligase BioID2 (MK-BioID2) conditioned medium for in-tube biotinylation assay
  • as a component of lysis buffer and amplified RNA (aRNA) fragmentation buffer to perform aRNA fragmentation
  • as a component of lysis buffer and DamID buffer for sequencing zygote samples

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL3788
BCCJ9123
BCCH2942
BCCH5826
BCCG7154

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Scientific reports, 8(1), 12671-12671 (2018-08-25)
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Analytical and bioanalytical chemistry, 408(29), 8343-8353 (2016-10-04)
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