16-209
Anti-GST Tag Antibody, clone DG122-2A7, HRP conjugate
clone DG122-2A7, Upstate®, from mouse
Synonym(s):
70 kDa zeta-associated protein, Syk-related tyrosine kinase, zeta-chain (TCR) associated protein kinase (70 kD), zeta-chain (TCR) associated protein kinase 70kDa, zeta-chain associated protein kinase 70kDa, zeta-chain associated protein kinase, 70kD
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.56
Recommended Products
biological source
mouse
Quality Level
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
DG122-2A7, monoclonal
species reactivity
Schistosoma japonicum
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
General description
Immunogen: GST (Glutathione S-transferase) derived from the pGEX expression vector and linked to KLH.
Specificity: Recognizes GST-fusion proteins.
Specificity: Recognizes GST-fusion proteins.
Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.
Specificity
Recognizes GST-fusion proteins.
Immunogen
GST (Glutathione S-transferase) derived from the pGEX expression vector and linked to KLH. Clone DG122-2A7.
Application
Anti-GST Tag Antibody, clone DG122-2A7, HRP conjugate detects level of GST Tag & has been published & validated for use in WB.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
Quality
Routinely evaluated by western blot Analysis on recombinant GST protein.
Western Blot Analysis:
1:500-1:1000 dilution of this lot detected recombinant GST protein.
Western Blot Analysis:
1:500-1:1000 dilution of this lot detected recombinant GST protein.
Physical form
Protein G Purified
Purified mouse monoclonal IgG in buffer containing (HRP) in 70% storage buffer PBS with 0.05% Kathon® and 30% glycerol. Liquid at -20°C.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Control
Cells expressing the GST-tag.
Cells expressing the GST-tag.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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