SCJ4600029

CF^™ 594 hydrazide

suitable for fluorescence, ≥90% (HPLC), ≥90% (TLC)

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• Quality Level: 100
• Assay: ≥90% (HPLC); ≥90% (TLC)
• form: solid
• mol wt: ~743 g/mol
• solubility: H₂O: soluble
• ε (extinction coefficient): 115000 at 593 nm
• absorbance ratio: A_{280/593 nm} 0.08
• fluorescence: λ_ex 532 or 561 nm (Flow cytometry laser line); λ_ex 593 nm; λ_em 614 nm; λ_ex 594 nm (Microscopy laser line)
• suitability: suitable for fluorescence
• storage temp.: −20°C

Description

General description

CF^™594 is a deep red fluorescent dye spectrally similar to Alexa Fluor^™ 594 and Texas Red^® dye. When conjugated to proteins, CF^™594 is significantly brighter than Alexa Fluor^™ 594 due to its high quantum yield and exceptional water solubility. CF^™594 also has excellent photostability, making it ideal for demanding applications such as confocal microscopy and single molecule imaging. These properties make CF^™594 the best deep-red dye for labeling proteins and nucleic acids. The dye is particularly useful in combination with our blue fluorescent CF^™350, green fluorescent CF^™488A and far red CF^™647 for multi-color imaging.

CF^™ dye hydrazides can be used as a fixable polar tracers for visualizing cell morphology,or for gap junction studies. The dyes can be introduced into cells by microinjection. For conjugation to aldehyde or ketone groups (such as carbohydrate molecules after peroxidation with periodate) we recommend using the more reactive CF aminooxy dye forms.

Features and Benefits

1. Yields the brightest antibody conjugates among spectrally similar dyes. 2. Extremely photostable. 3. Highly water-soluble.

Preparation Note

Protect from light

Reconstitution

Dissolve in water

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.

ALEXA FLUOR is a trademark of Life Technologies

CF is a trademark of Biotium, Inc.

Texas Red is a registered trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable