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O4387

Sigma-Aldrich

Oligo(dT)23, Anchored

70 μM in H2O

Synonym(s):

Oligo(dT)23 Anchored Primer

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About This Item

UNSPSC Code:
41106305
NACRES:
NA.52

biological source

synthetic (organic)

form

solution

usage

0.1 mL sufficient for 100 RT-PCR reactions (as described in the Technical Bulletin for Product Codes HSRT100 and HSRT20)

concentration

70 μM in H2O

color

colorless

shipped in

wet ice

storage temp.

−20°C

General description

Oligo(dT)23, Anchored primers are used to prime mRNA with a poly(A) tail for cDNA synthesis. The primers have 23 thymidine residues and one G, C, or A residue (the anchor) at the 3′ end. This anchor ensures that the oligo(dT) primer binds at the very beginning of the message and there is not a long region of the unusable sequence. The optimal conditions for the concentration of enhanced AMV reverse transcriptase, template RNA, primers, and amplification parameters will depend on the system being utilized and should be determined empirically.

Application

Oligo(dT)23, Anchored has been used in the synthesis of cDNA for real-time quantitative polymerase chain reaction (PCR).
The Anchored Oligo(dT)23 Primers have 23 thymidine residues and one G, C, or A residue (the anchor) at the 3′ end. This anchor ensures the oligo(dT)23 primer binds at the beginning of the message such that there are no long regions of unusable sequence. Anchored oligo(dT)23 primers may provide an advantage over standard oligo(dT) primers when generating cDNA from poly(A)+ RNA.

Features and Benefits

  • In the process of preparing cDNA libraries, when there is incomplete or missing sequence information or when specific primers are not suitable, Oligo(dT)23, Anchored primers can be employed together with random nonamers (Product No. R 7647).
  • These primers can serve as a substitute for reverse transcription primers in tasks like first-strand cDNA synthesis, cDNA library construction, and various other applications.
  • Following transcription, the anchored oligo(dT)23 primers exhibit a reduced ability to initiate priming at higher temperatures (up to 65 °C), ensuring that they do not interfere with PCR.
  • Anchored oligo(dT)23 primers may provide an advantage over standard oligo(dT) primers when generating cDNA from poly(A)+ RNA.

Other Notes

For laboratory use only. Not for drug, household or other uses.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Fiona S McDonnell et al.
Journal of glaucoma, 25(10), e834-e842 (2016-10-18)
Glaucoma is an optic neuropathy that affects 60 million people worldwide. There is an underlying fibrosis associated with the lamina cribrosa (LC) in glaucoma. DNA methylation is well established in regulating fibrosis and may be a therapeutic target for glaucoma.
Leila Azimi et al.
Gene, 576(1 Pt 1), 166-170 (2015-10-13)
Carbapenemase production causes multi antibiotics resistant in Gram-negative bacteria. A simple rapid and accurate phenotypic test for detection of Gram-negative carbapenemase-producing bacteria is useful for the treatment of infections. The aim of this study was to track the negative results
Christian Fork et al.
Journal of lipid research, 58(2), 386-392 (2016-12-04)
Nonsteroidal anti-inflammatory drugs are the most widely used medicine to treat pain and inflammation, and to inhibit platelet function. Understanding the expression regulation of enzymes of the prostanoid pathway is of great medical relevance. Histone acetylation crucially controls gene expression.
P Goelet et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(19), 5818-5822 (1982-10-01)
Oligonucleotide primers have been used to generate a cDNA library covering the entire tobacco mosaic virus (TMV) RNA sequence. Analysis of these clones has enabled us to complete the viral RNA sequence and to study its variability within a viral
Sigma's New Enhanced Avian RT-PCR Kit.
Eastlund, E. and Song, K.
Sigma data, 1, 15-17 (2000)

Articles

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.

Protocols

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.

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