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A2856

Sigma-Aldrich

Anti-ATG7 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

ATG7 Antibody - Anti-ATG7 antibody produced in rabbit, Atg7 Antibody, Anti-APG7, Anti-APG7-like, Anti-APG7L, Anti-Apoptosis-specific-protein, Anti-Autophagy protein 7-like, Anti-Ubiquitin activating enzyme E1-like protein

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About This Item

MDL number:
MFCD09753848
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~75 kDa

species reactivity

rat, human, mouse

packaging

antibody small pack of 25 μL

technique(s)

immunoprecipitation (IP): 1-2 μL using human U87 cell lysate
western blot: 0.5-1 μg/mL using whole extracts of mouse 3T3 cells

UniProt accession no.

O95352

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ATG7(10533)
mouse ... Atg7(74244)
rat ... Atg7(312647)

General description

Autophagy is a catabolic process by which cell delivers cytoplasmic constituent into lysosome for protein degradation. 16 ATG genes are essential for autophagosome preparation out of which Atg7 is the one required to activate ubiquitin-like proteins Atg12 and Atg8. Atg7 is also crucial for amino acid supply in neonates, as well as for bulk degradation of proteins and organelles in mice during starvation. Anti-ATG7 antibody can be used in immunohistochemistry and immunoprecipitation. Rabbit anti-ATG7 antibody reacts specifically with Atg7 of human, mouse and rat.

Immunogen

synthetic peptide corresponding to amino acids 38-50 of human ATG7, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in mouse and rat.

Application

Anti-ATG7 antibody can be used in western blotting and immunoblotting.
Anti-ATG7 antibody produced in rabbit has been used:
  • for quantitative real-time polymerase chain reaction of autophagy-related genes
  • in immunofluorescence and immunoblotting
  • in in vivo acetylation/deacetylation of autophagy proteins

Rabbit anti-ATG7 antibody has been used for immunoblotting assays. The antibody is also suitable for use in immunoprecipitation applications at 1-2μL using human U87 cell lysates.
Rabbit anti-ATG7 antibody was used for immunohistochemistry on sections of mouse midbrain fixed in 4% paraformaldehyde at 1:2000.

Biochem/physiol Actions

Autophagy related 7 (ATG7) regulates caspase mediated autophagy and apoptosis. This has a significant effect on tumor progression and ATG7 acts as a tumor suppressor. ATG7 is known to be associated with many cancer types, hence it is considered as a target for cancer therapy. Loss of ATG7 gene contributes to liver carcinogenesis. The ATG7 gene encodes E1-like enzyme essential for lipidation and conjugation systems : ubiquitin-like microtubule-associated protein 1A/1B-light chain 3 (LC3) and autophagy related 12 (ATG12) respectively.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Altea Rocchi et al.
PLoS genetics, 13(8), e1006962-e1006962 (2017-08-15)
Impairment of the autophagy pathway has been observed during the pathogenesis of Alzheimer's disease (AD), a neurodegenerative disorder characterized by abnormal deposition of extracellular and intracellular amyloid β (Aβ) peptides. Yet the role of autophagy in Aβ production and AD
miR-106a suppresses tumor cells death in colorectal cancer through targeting ATG7
Hao H, et al.
Medical Molecular Morphology, 50(2), 76-85 (2017)
Autophagy protects against colitis by the maintenance of normal gut microflora and mucus secretion
Tsuboi K, et al.
The Journal of Biological Chemistry (2015)
Christian Pohl et al.
Nature cell biology, 11(1), 65-70 (2008-12-17)
At the end of cytokinesis, the dividing cells are connected by an intercellular bridge, containing the midbody along with a single, densely ubiquitylated, circular structure called the midbody ring (MR). Recent studies revealed that the MR serves as a target
ATG7 regulates energy metabolism, differentiation and survival of Philadelphia-chromosome-positive cells
Karvela M, et al.
Autophagy, 12(6), 936-948 (2016)
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