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MABF2058

Sigma-Aldrich

Anti-NKp46 (CD335) Antibody, clone D2-9A5

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

antibody form

purified antibody

clone

D2-9A5
monoclonal

purified by

using protein G

species reactivity

mouse, human, human

concentration

(Please refer to lot specific datasheet.)

technique(s)

ELISA: suitable
flow cytometry: suitable

isotype

IgG1κ

UniProt accession no.

General description

Human IgG1-Fc - fused to a recombinant fragment corresponding to 135 amino acids from the ectodomain region of human NKp46.

Specificity

Clone D2-9A5 recognizes the whole NKp46 and NKp46-D2, but does not recognize NKp46-D1. It targets an epitope with in 135 amino acids from the extracellular domain.

Application

Flow Cytometry Analysis: A representative lot detected NKp46 (CD335) in Flow Cytometry applications (Shemer-Avni, Y., et. al. (2017). Front Immunol. 8:161).

Flow Cytometry Analysis: 4 µg/mL from a representative lot detected NKp46 (CD335) in NK92 cells transfected with human NKp46 (Courtesy of Angel Porgador, Ph.D., Faculty of Health Sciences, Ben Gurion university of the Negev, Israel).

ELISA Analysis: A representative lot detected NKp46 (CD335) in ELISA applications (Shemer-Avni, Y., et. al. (2017). Front Immunol. 8:161).

Quality

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.

Physical properties

34.48 kDa calculated.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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