R1003

Ribonuclease T₁ from Aspergillus oryzae

ammonium sulfate suspension, 300,000-600,000 units/mg protein

• Synonym(s): Guanyloribonuclease, Ribonucleate 3′-guanylo-oligonucleotidohydrolase
• CAS Number: 9026-12-4
• Enzyme Commission number: 3.1.27.3 (BRENDA, IUBMB)
• EC Number: 232-809-1
• MDL number: MFCD00132191
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Aspergillus sp. (Aspergillus oryzae)
• form: ammonium sulfate suspension
• specific activity: 300,000-600,000 units/mg protein
• mol wt: 11068 by amino acid sequence
• technique(s): cell based assay: suitable
• suitability: suitable for separating native or denatured proteins, or nucleic acids
• application(s): cell analysis
• storage temp.: 2-8°C

Description

Application

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies .

Biochem/physiol Actions

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3′-phosphate group of a guanidine ribonucleotide and 5′-hydroxyl of the adjacent nucleotide. The initial product is a 2′:3′ cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3′-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3′-GMP and oligonucleotides with a 3′-GMP terminal group.

Unit Definition

One unit will produce acid soluble oligonucleotides equivalent to a ΔA₂₆₀ of 1.0 in 15 min at pH 7.5 at 37°C, in a reaction volume of 1.0 mL. Substrate: Yeast RNA.

Physical form

Suspension in 2.8 M (NH₄)₂SO₄ solution

Analysis Note

Protein determined by E1%/280

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves