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I7633

IMDM

With ʟ-glutamine and HEPES, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217469
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Product Name

Iscove′s Modified Dulbecco′s Medium, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

components

phenol red: yes
glucose: yes
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

General description

Iscove′s Modified Dulbecco′s medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids, and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate. Iscove′s medium supports murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Application

Iscove′s Modified Dulbecco′s Medium has been used to culture KG1a cells (human leukemic cell line)[1][2] and Chinese hamster ovary (CHO) DG44 cell line.[3]
Reported to support murine B lymphocytes, hematopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Preparation Note

Formulated to contain 17.7 grams of powder per liter of medium.
Supplement with 3.024 g/L sodium bicarbonate.

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This Item
I652951471CI3390
Iscove’s Modified Dulbecco’s Medium With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

I7633

Iscove′s Modified Dulbecco′s Medium

Iscove’s Modified Dulbecco’s Medium With sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma

SAFC

I6529

Iscove′s Modified Dulbecco′s Medium

Iscove’s Modified Dulbecco’s Medium liquid

Sigma-Aldrich

51471C

Iscove′s Modified Dulbecco′s Medium

Iscove’s Modified Dulbecco’s Medium liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma

Sigma-Aldrich

I3390

Iscove′s Modified Dulbecco′s Medium

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

form

powder

form

liquid

form

liquid

form

liquid

components

phenol red: yes
glucose: yes
L-glutamine: yes

components

glucose: yes
phenol red: yes
NaHCO3: yes
L-glutamine: yes

components

phenol red: 15.93 mg/L
NaHCO3: 3024 mg/L
L-glutamine: 582 mg/L
HEPES: 5958 mg/L
sodium pyruvate: 110 mg/L

components

phenol red: yes
L-glutamine: no
glucose: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

classic media, classical media, FBS, lymphocyte cell culture, hematopoietic cell culture

classic media, classical media, serum supplement, FBS, embryonic mouse cells, high-density cultures, rapidly proliferating, synthetic media

classic media, classical media, serum supplement, FBS, embryonic mouse cells, high-density cultures, rapidly proliferating, synthetic media

classic media, classical media, FBS, hematopoietic cells

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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000500463
0000500465
0000500465
0000500463
0000500460

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Ryan S Pawell et al.
Biomicrofluidics, 7(5), 56501-56501 (2014-01-10)
Deterministic lateral displacement (DLD) is a microfluidic size-based particle separation or filter technology with applications in cell separation and enrichment. Currently, there are no cost-effective manufacturing methods for this promising microfluidic technology. In this fabrication paper, however, we develop a
Yogesh Kumar Verma et al.
International journal of radiation biology, 96(5), 671-688 (2020-01-28)
Purpose: Lithium chloride (LiCl) is clinically used for manic disorders. Its role has been shown in improving cell survival by decreasing Bax and p53 expression and increasing Bcl-2 concentration in the cell. This potential of LiCl is responsible for reducing
Hale M Tasyurek et al.
Human gene therapy, 29(7), 802-815 (2018-02-08)
Postprandial glucose-induced insulin secretion from the islets of Langerhans is facilitated by glucagon-like peptide-1 (GLP-1)-a metabolic hormone with insulinotropic properties. Among the variety of effects it mediates, GLP-1 induces delta cell secretion of somatostatin, inhibits alpha cell release of glucagon
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Global Trade Item Number

SKUGTIN
I7633-10X1L04061833858332
I7633-BIBC04061826573730
I7633-50K04061834180449
I7633-10L04061833858325
I7633-50L04061833858349

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