The 100x stock solution of this product has a 2-year shelf life when stored at 2-8°C. However, stability of the solution after dilution or after opening has not been tested.
I3146
ITS Liquid Media Supplement (100×)
liquid, sterile-filtered, BioReagent, suitable for cell culture
Synonym(s):
Human ITS Liquid Media, Insulin-transferrin-sodium selenite
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352205
Pricing and availability is not currently available.
sterility
sterile-filtered
Quality Segment
product line
BioReagent
form
liquid
technique(s)
cell culture | mammalian: suitable, single cell analysis: suitable
impurities
endotoxin, tested
shipped in
ambient
storage temp.
2-8°C
Application
Biochem/physiol Actions
Preparation Note
Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
Other Notes
Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.
ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.
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This Item | |||
|---|---|---|---|
| form liquid | form liquid | form liquid | form solution |
| technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| Quality Level 500 | Quality Level 500 | Quality Level 300 | Quality Level 400 |
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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培地で希釈した1×Solutionは冷蔵でどのくらい保管できますか？
1 answer-
Helpful?
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Can this ITS be diluted in DMEM medium containing phenol red
1 answer-
This product is compatible with DMEM containing phenol red.
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What should you use to dilute this to a 1X solution?
1 answer-
This product can be dissolved in a given target cell culture medium, e.g., non-complex media (MEM, RPMI-1640), to a 1X solution.
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What are the product numbers for the stand alone components in I3146?
1 answer-
The product numbers for the stand-alone components in I3146 are as follows:
Insulin – I2643 or I3536 (sterile)
Apo-Transferrin – T2252
Sodium Selenite – S5261
EBBS – E3024Helpful?
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The ITS product #I3146 has been on back order for a while. Is there an alternative with similar components?
1 answer-
Based on the Media Supplement Formulation, the closest alternative to product No. I3146 is the I2521 ITS+1 Liquid Media Supplement (100×), which also contains BSA and Linoleic acid.
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