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F8146

Sigma-Aldrich

Anti-Factor VII antibody, Mouse monoclonal

clone HVII-1, purified from hybridoma cell culture

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HVII-1, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

indirect ELISA: suitable
western blot: 1:4,000

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... F7(2155)

General description

Anti-Factor VII antibody, mouse monoclonal (mouse) IgG1 isotype) is derived from the HVII-1 hybridoma produced by the fusion of mouse Sp2/0-Ag14 myeloma cells and splenocytes from immunized BAB/c mice. Human coagulation factor VII contains 10 gamma-carboxyglutamic acid residues (Gla), located at the N-terminal region of the molecule. The activated factor VII (factor VIIa) also consists of a light chain with the Gla-domain and an epidermal growth factor domain, which is linked by a disulfide bond to a heavy chain, containing the serine protease catalytic domain.

Specificity

The antibody recognizes an epitope on the light chain of Factor VII in the presence or absence of divalent cations. Clone no. HVII-1 (also cited as clone no. MC1476/E.A.8.1) may be used for purification of Factor VII and for the preparation of Factor VII depleted human plasma.

Immunogen

Factor VII from pooled normal human plasma.

Application

Anti-Factor VII antibody, Mouse monoclonal has been used in:
  • the preparation of factor VII - depleted plasma and for purification
  • sandwich-type immunoassays
  • the evaluation of patients with hereditary factor VII deficiency by an enzyme linked immunosorbent assay (ELISA) or radio immunosorbent assay (RIA)
  • the assay of factor VII level in patients with liver disease (a sensitive parameter of liver dysfunction)
  • epidemiological studies of the importance of factor VII level as a risk factor for coronary heart disease, cerebrovascular disease and peripheral vascular diseases
  • the determination of the activity state of factor VII in in vivo samples when used with a clotting assay
  • flow cytometry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Monoclonal Anti-Factor VII antibody is suitable for flow cytometry analysis to study the behavior of TF-fVIIa secreted by ovarian cancer cells was associated with microparticles (MPs). It is also suitable for western blot at a dilution of 1:4,000 and indirect ELISA.

Biochem/physiol Actions

Factor VII is a single-chain vitamin K-dependent glycoprotein in human coagulation. It has a molecular weight of ∼50,000Da, present in plasma in trace quantities. It is predominantly synthesized in liver and depends on the posttranslational γ−carboxylation of specific glutamic residues located near the NH2-terminus of the molecule. It is a serine protease zymogen and for activation it requires proteolytic cleavage in the disulfide-linked, two-chain form, Factor VIIa, by Factor Xa, Factor IXa, thrombin and Factor XIIa or β XIIa.

Physical form

Solution in 10 mM HEPES buffer, pH 7.4, with 140 mM sodium chloride and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Regulated readthrough: A new method for the alternative tagging and targeting of recombinant proteins
Bouquin T, et al.
Journal of Biotechnology, 125(4), 516-528 (2006)
Structural biology of factor VIIa/tissue factor initiated coagulation
Vadivel K and Bajaj SP
Frontiers in Bioscience, 17, 2476-2476 (2012)
G J Broze et al.
The Journal of biological chemistry, 255(4), 1242-1247 (1980-02-25)
Blood coagulation Factor VII was purified 100,000-fold from fresh frozen human plasma to apparent homogeneity with a yield of 30% based on coagulation assay. The molecular weight estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was 48,000. Factor VII is composed
Self-production of tissue factor-coagulation factor VII complex by ovarian cancer cells
Yokota N, et al.
British Journal of Cancer, 101(12), 2023-2023 (2009)
N Yokota et al.
British journal of cancer, 101(12), 2023-2029 (2009-11-12)
Thromboembolic events are a major complication in ovarian cancer patients. Tissue factor (TF) is frequently overexpressed in ovarian cancer tissue and correlates with intravascular thrombosis. TF binds to coagulation factor VII (fVII), changing it to its active form, fVIIa. This

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