The formulation of this product is considered proprietary. However, propylene glycol and glycerol are added as stabilizers.
C2605
Cellulase from Aspergillus sp.
aqueous solution
Sustainability features
Synonym(s):
Carezyme®
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-734-4
MDL number:
EC Number:
eCl@ss:
32160410
Specific activity:
≥1000 units/g
Pricing and availability is not currently available.
Quality Segment
form
solid
specific activity
≥1000 units/g
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Sustainability features, Sustainability features
storage temp.
2-8°C
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research
Application
Cellulase from Aspergillus sp. has been used to degrade cello-oligosaccharides into glucose during the analysis of BBC (bioabsorbable bacterial cellulose) biodegradability. The enzyme has also been used in photon correlation spectroscopy to study its effects on unfiltered beer samples. The parameters evaluated in this spectroscopic analysis were the size of suspended particles, flow rate and haze stability.
Biochem/physiol Actions
Cellulases are enzymes that hydrolyze cellulose, a linear polymer of anhydroglucose units linked together by β-1,4-glycosidic bonds, to glucose. Endo-β-D-glucanase is one of the major component enzymes of the cellulase complex. It catalyzes the hydrolysis of cellulose by randomly splitting the sugar residues within the molecule. Exo-β-D-glucanase and β-glucosidase can synergistically convert cellulose into glucose and hence are used on an industrial scale.
Preparation Note
produced by submerged fermentation of a genetically modified Aspergillus microorganism
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Legal Information
A product of Novozyme Corp.
Carezyme is a registered trademark of Novozymes Corp.
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This Item | |||
|---|---|---|---|
| description aqueous solution | description lyophilized powder, ≥1 unit/mg solid | description aqueous solution, ≥3,800 units/mL | description powder, ~1.5 U/mg (~0.2 U acc. to Willstätter) |
| specific activity ≥1000 units/g | specific activity ≥1 unit/mg solid | specific activity ≥3,800 units/mL | specific activity ~1.5 U/mg (~0.2 U acc. to Willstätter) |
| form solid | form lyophilized powder | form aqueous solution | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
| greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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is this formulation contain glycerol as stabilizer?
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What is the total protein content (% w/w or mg/mL) of the liquid formulation?
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This is a product of Novozyme Corp. The protein content is not among its release specifications. Its efficacy is determined by its enzymatic activity in terms of units per gram liquid. To get a units/mL value, one can use the density value of approximately 1.2 g/mL to convert to a units/mL value for each lot.
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what is enzyme activity in FPU for C2605
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Unfortunately, the enzyme activity is not tested in terms of filter paper units (FPU). It is presented as U/g.
Kindly review the following citation that provides a reference to the FPU method (Adney and Baker). As this value is not presented on the certificate of analysis, it may be necessary to re-assess the FPU for every batch received:
Rodrigues AC, Haven MØ, Lindedam J, Felby C, Gama M. Celluclast and Cellic® CTec2: Saccharification/fermentation of wheat straw, solid-liquid partition and potential of enzyme recycling by alkaline washing. Enzyme Microb Technol. 2015 Nov;79-80:70-7. doi: 10.1016/j.enzmictec.2015.06.019. Epub 2015 Jul 17. PMID: 26320717.
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What is the shelf life of the Cellulase from Aspergillus sp.?
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This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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What is the optimum pH for Cellulase from Aspergillus sp., Product C2605?
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At a temperature of 40°C, Novozyme has found ~pH 8.0-8.2 to be the optimum pH range for this enzyme.
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What is the optimum temperature for activity for Cellulase from Aspergillus sp., Product C2605?
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At pH 8.5, Novozyme has determined that the optimal activity for this enzyme is ~60°C. The percentage of maximum activity is ~40% of that value at 40°C, and ~80% at 50°C. There is a steep drop in activity above 60°C.
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What are the supplier's working conditions for determining the activity for Product C2605, Cellulase from Aspergillus sp.?
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This product is assayed using carboxymethycellulose as substrate, at 40°C, at a pH of 7.5.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the density of Product C2605, Cellulase from Aspergillus sp.?
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The density of this cellulase solution is approximately 1.2 g/mL.
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How are the activity units of Product C2605, Cellulase from Aspergillus sp., listed on the C of A?
1 answer-
The activity of cellulase given on the C of A is in units per gram of liquid.
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