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A1960

Aggrecan from bovine articular cartilage

lyophilized powder

Synonym(s):

Aggrecan Protein

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.75
MDL number:
MFCD00676441
Form:
lyophilized powder
Biological source:
bovine articular cartilage
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biological source

bovine articular cartilage

Quality Level

200

form

lyophilized powder

packaging

glass bottle of 1 mg

technique(s)

cell culture | mammalian: suitable

impurities

salt, essentially free

color

white

solubility

H2O: 2 mg/mL

UniProt accession no.

P13608

shipped in

wet ice

storage temp.

−20°C

Gene Information

cow ... AGC1(280985)

Application

Aggrecan is a critical component for cartilage structure and the function of joints. Its synthesis and degradation are being studied for their roles in cartilage deterioration during joint injury disease and aging.

Biochem/physiol Actions

This molecule produces a rigid, reversibly deformable gel that resists compression. It combines with hyaluronic acid to form very large macromolecular complexes that have an increased hydrohynamic volume and a significant increase (30-40%) in the relative viscosity of the solution.

Preparation Note

This product is extracted from articular cartilage, chematographically purified, dialyzed against water, and 0.2 μm filtered prior to lyophilization. Once lyophilized, this powder is essentially salt-free. The product is soluble in water at 2 mg/mL.

Other Notes

Aggrecan is a major structural proteoglycan of the cartilage extracellular matrix. It is a large proteoglycan, with a molecular weight greater than 2,500 kDa composed of approximately 100-150 glycosaminoglycan chains attached to a core protein. The majority of these glycosaminoglycan chains are chondroitin/dermatan sulfate. Aggrecan contains three globular domains, G1, G2, and G3, which are involved in aggregation and hylauronan binding, cell adhesion and chondrycte apoptosis.

Disclaimer

Store this product at -20°C. Stored as supplied, this powder shows little decomposition in 3 years when stored properly.

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Aggrecan from bovine articular cartilage lyophilized powder

Sigma-Aldrich

A1960

Aggrecan from bovine articular cartilage

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Sigma-Aldrich

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Sigma-Aldrich

A4161

Bovine Serum Albumin

Calmodulin from bovine testes BioUltra, ≥98% (SDS-PAGE), lyophilized powder, essentially salt free

Sigma-Aldrich

P1431

Calmodulin from bovine testes

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

ligand binding assay: suitable

biological source

bovine articular cartilage

biological source

-

biological source

bovine

biological source

bovine testis

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

impurities

salt, essentially free

impurities

salt, essentially free

impurities

essentially globulin free

impurities

salt, essentially free

solubility

H2O: 2 mg/mL

solubility

PBS: 5 mg/mL, slightly hazy, H2O: soluble, slightly hazy

solubility

water: soluble (50 mg/ml)

solubility

-

Gene Information

cow ... AGC1(280985)

Gene Information

-

Gene Information

bovine ... ALB(280717)

Gene Information

cow ... CALM3(520277)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000354979
0000354979
0000180333
0000180333
0000114095

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Related Content

L Cao et al.
Matrix biology : journal of the International Society for Matrix Biology, 17(5), 379-392 (1998-11-20)
The proteoglycan aggrecan is a major component of cartilage, and degradation of aggrecan is associated with aging and a number of pathological conditions. To investigate the effects of the accumulation of G1 domain from degraded aggrecan, we overexpressed the G1
C J Billington et al.
The Biochemical journal, 336 ( Pt 1), 207-212 (1998-11-10)
The breakdown of aggrecan in cartilage is, in part, mediated by an enzyme named aggrecanase that cleaves within the interglobular domain of the molecule between a glutamic residue and an alanine residue. Although the enzyme cleavage site has been identified
H Hedlund et al.
The Journal of biological chemistry, 274(9), 5777-5781 (1999-02-20)
Aggrecan, the predominant large proteoglycan of cartilage, is a multidomain macromolecule with each domain contributing specific functional properties. One of the domains contains the majority of the keratan sulfate (KS) chain substituents and a protein segment with a proline-rich hexapeptide
View All Related Papers

Global Trade Item Number

SKUGTIN
A1960-1MG04061833343258

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