Key Documents

Cholesterol Esterase from porcine pancreas

Name: Cholesterol Esterase from porcine pancreas
Brand: SIGMA

lyophilized, powder, white, ~35 U/mg

Synonym(s):

bile salt-dependent lipase, bile salt-stimulated lipase, carboxyl ester lipase, nonspecific lipase, pancreatic lysophospholipase, Cholesterol Esterase from hog pancreas, Sterol-ester acylhydrolase

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About This Item

CAS Number:

9026-00-0

UNSPSC Code:

12352204

NACRES:

NA.77

EC Number:

232-808-6

MDL number:

MFCD00071052

EC Number:

3.1.1.13 (BRENDA, IUBMB)

Specific activity:

~35 U/mg

Biological source:

Porcine pancreas

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Properties

biological source

Porcine pancreas

Quality Segment

100

form

powder

quality

lyophilized

specific activity

~35 U/mg

mol wt

M_r ~440000

color

white

storage temp.

−20°C

Description

General description

Research area: Cell signaling

Pancreatic cholesterol esterase (CEase), also known as bile salt stimulated lipase, is a serine hydrolase belonging to the α/β hydrolase family of enzymes. They are released from the exocrine pancreas. Serine 194, histidine 435 and aspartate 320 represent the catalytic triad of the enzyme.

Application

Cholesterol Esterase from porcine pancreas has been used:

in an in vitro simulated digestion model to improve the hydrolysis of carotenoid esters in the intestinal phase
to analyze the bioavailability of phytosterol and cholesterol in the aqueous micellar phase of an in vitro simulated digestion model
as a standard in enzyme activity assay

Biochem/physiol Actions

Cholesterol esterase (CEase), an enzyme having broad substrate specificity plays a key role in regulating the bile salt mediated hydrolysis of dietary cholesteryl esters. It also participates in the hydrolysis of triglycerides and phospholipids which are essential for normal cholesterol absorption. Cholesterol esterase hydrolysis helps in the determination of total cholesterol levels in the serum and plasma. Additionally, cholesterol esterase (CEase) along with phospholipase A2 is involved in the hydrolysis of lecithin to lysolecithin, forming intestinal micelles that deliver free cholesterol to enterocytes. Loss of enzyme function results in reduced intestinal absorption of dietary cholesteryl esters and prevents the formation of intestinal micelles, thereby failing to deliver cholesterol efficiently. Circulating cholesterol esterases, accumulated in atherosclerotic lesions are involved in triggering the proliferation of smooth muscle cells. Lastly, they are also selectively involved in the hydrolysis/condensation of carboxylic ester bonds.

Other Notes

1 U corresponds to the amount of enzyme which liberates 1 μmol cholesterol per minute at pH 7.0 and 37°C (cholesterol acetate as substrate)

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This Item	C3766	46058	E0258
Sigma-Aldrich 26745 Cholesterol Esterase from porcine pancreas	Sigma-Aldrich C3766 Cholesterol Esterase from bovine pancreas	Sigma-Aldrich 46058 Esterase from porcine liver	Sigma-Aldrich E0258 Elastase from porcine pancreas
description lyophilized, powder, white, ~35 U/mg	description lyophilized powder, ≥200 units/g protein	description lyophilized, powder, slightly beige, ≥50 U/mg	description Type IV, Protein 50-90 %, lyophilized powder, ≥4.0 units/mg protein (biuret)
specific activity ~35 U/mg	specific activity ≥200 units/g protein	specific activity ≥50 U/mg	specific activity ≥4.0 units/mg protein (biuret)
biological source Porcine pancreas	biological source -	biological source Porcine liver	biological source Porcine pancreas
form powder	form lyophilized powder	form lyophilized solid, powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt M_r ~440000	mol wt 84 kDa by SDS-PAGE	mol wt M_r ~162000	mol wt -
color white	color -	color slightly beige	color -

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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