EZHIL8

Human IL-8 ELISA Kit

detect IL-8 in 50 μL serum, plasma or cell culture supernatent

• Synonym(s): Granulocyte chemotactic protein 1, IL-8, Interleukin-8, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, T-cell chemotactic factor
• UNSPSC Code: 41116158
• NACRES: NA.84
• Gene information: human ... CXCL8(3576)
• Input: sample type plasma (K2 EDTA); sample type cell culture supernatant; sample type serum
• Species reactivity: human
• Storage temp.: 2-8°C

Properties

• Product Name: Human IL-8 Conferma^® ELISA, quantification of Human IL-8 in serum, plasma, or culture media samples
• Quality Segment: 200
• species reactivity: human
• parameter: 50 μL sample volume (4hr assay)
• technique(s): ELISA: suitable
• input: sample type plasma (K2 EDTA); sample type cell culture supernatant; sample type serum
• UniProt accession no.: P10145
• application(s): research use
• assay range: accuracy: 77-102 (plasma)%; linearity: 105 (plasma)%, inter-assay cv: <4.7%; intra-assay cv: <4.0%; standard curve range: 0.82-200 pg/mL; accuracy: 70-105 (serum)%; linearity: 94-106 (serum)%
• detection method: colorimetric (450nm/590nm)
• storage temp.: 2-8°C
• Gene Information: human ... CXCL8(3576)

Description

General description

IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. IL-8 also participates with other cytokines in the proinflammatory signaling cascade. The protein is implicated in numerous pathologies including, bacterial, cancer, cardiovascular and viral diseases.
IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.

This is a 96 well format sandwich ELISA which uses our “Conferma” process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots.

Application

This Human Interleukin-8 (IL-8) ELISA kit is used for the quantification of Human IL-8 in serum, plasma, or culture media samples.

Analytes available:

• IL-8

Research Category:

• Cytokines
• Inflammation & Immunology
• Immune Response
• Metabolic Disease

Analysis Note

3 hour 45 minute assay, room temperature, 50μl of neat serum or plasma is recommened.

Legal Information

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For research use only. Not for use in diagnostic procedures.