The type designations for urease products are not indications of particular isoforms. They are internal codes that refer to the chronological order of the products' market entry and manufacturing processes. The manufacturing processes are proprietary. However, the major differences between the urease products are their specifications for specific activity. Please access the 'Specification Sheet' and 'Sample Certificate of Analysis' for each individual product via its corresponding website.
U4002
Urease from Canavalia ensiformis (Jack bean)
Type IX, powder, 50,000-100,000 units/g solid
Synonym(s):
Jack bean urease, Urea amidohydrolase
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About This Item
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type
Type IX
Quality Level
form
powder
specific activity
50,000-100,000 units/g solid
mol wt
~544620 Da
storage temp.
2-8°C
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General description
Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Composed of six subunits with total molecular weight: ~544,620
Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C.[1] Urease binds to nickel and is present in archaea, bacteria, unicellular eukaryotes and plants.[2] Jack bean urease was the first enzyme to be crystallized and the first enzyme found to contain nickel.[3] It is a multi-subunit enzyme, consisting of 91 kDa subunits in three protein forms.
Application
Biochem/physiol Actions
In Canavalia ensiformis (Jack Bean), urease facilitates the conversion of L-canavanine to canaline. [6]
Urease is involved in purine metabolism and the urea cycle. It hydrolyze urea to produce ammonia and carbon dioxide.[7]
Unit Definition
One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
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Good morning, I would like to know what the difference is between the different types of urease, since some are type III, type IX, type C-III.
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