S1451

Anti-Sox2 antibody, Mouse monoclonal

clone SOX2-6, purified from hybridoma cell culture

• Synonym(s): Anti-ANOP3, Anti-MCOPS3, Anti-SRY (sex determining region Y)-box 2, Anti-SRY-related HMG-box gene 2, Anti-Sex-determining region Y-box 2
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: SOX2-6, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~35 kDa
• species reactivity: human, mouse
• concentration: ~1.0 mg/mL
• technique(s): western blot: 0.5-1.0 μg/mL using whole extract of human NT2 cells
• isotype: IgG1
• UniProt accession no.: P48431
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SOX2(6657) ; mouse ... Sox2(20674) ; rat ... Sox2(499593)

Description

General description

Monoclonal Anti-SOX2 (mouse IgG1 isotype) is derived from the hybridoma SOX2-6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice.

SOX2 (sex-determining region Y-box 2) is a transcription factor that is made of 317 amino acid. It has a HMG (high mobility group box) domain and is a critical transcription regulator of normal stem cell. It is located on human chromosome 3q26.3.

Application

Anti-Sox2 antibody has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.

SOX2 (sex-determining region Y-box 2) down-regulation reduces the stem cell count, that affects the breast cancer cells in initiating tumor progression. It participates in chemoresistance and regular lung cancer therapies. In mouse, SOX2 plays an important role in branching morphogenesis and regulating lung epithelial cell differentiation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable