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N7265

Sigma-Aldrich

Nitrate Reductase (NAD[P]H) from Aspergillus niger

lyophilized powder, ≥300 units/g solid

Synonym(s):

NAD(P)H:Nitrate oxidoreductase

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

specific activity

≥300 units/g solid

shipped in

wet ice

storage temp.

−20°C

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General description

Nitrate reductase (NAD[P]H) is expressed in variety of organisms including bacteria, fungi, algae, and higher plants which have capacity to use nitrate as their sole source of nitrogen.Experimental studies show that nitrate reductase (NAD[P]H) is 180,000Daltons.

Biochem/physiol Actions

Nitrate Reductase (NAD[P]H) catalyzes the reduction of nitrate to nitrite. In addition, it also catalyzes the reduction of NADPH-cytochrome c, FADH2-nitrate. Nitrate reductase also has reduced methyl viologen-nitrate reductase activities.

Caution

light and moisture sensitive

Unit Definition

One unit will reduce 1.0 μmole of nitrate per min in the presence of β-NADPH at pH 7.5 at 25 °C.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Bharat Bhushan et al.
Environmental science & technology, 36(14), 3104-3108 (2002-07-27)
Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) can be efficiently mineralized with anaerobic domestic sludge, but the initial enzymatic processes involved in its transformation are unknown. To test the hypothesis that the initial reaction involves reduction of nitro group(s), we designed experiments to test the
N Minagawa et al.
Journal of biochemistry, 91(3), 761-774 (1982-03-01)
NADPH-nitrate reductase [NADPH : nitrate oxidoreductase, EC 1.6.6.3] was purified 500-fold from Aspergillus nidulans with an overall yield of about 20%. The purified enzyme catalyzed NADPH-nitrate, NADPH-cytochrome c, FADH2-nitrate and reduced methyl viologen-nitrate reductase activities. Its molecular weight was estimated
E Fernández et al.
Proceedings of the National Academy of Sciences of the United States of America, 86(17), 6449-6453 (1989-09-01)
The nitrate reductase structural gene of Chlamydomonas reinhardtii has been isolated from a genomic library by using a nitrate reductase cDNA probe from barley. Restriction fragment length polymorphism analyses mapped the Chlamydomonas clone (B6a) to the nitrate reductase structural gene
Nilesh Dharajiya et al.
The Journal of allergy and clinical immunology, 119(3), 646-653 (2007-03-06)
Ragweed extract (RWE) contains NADPH oxidases that induce oxidative stress in the airways independent of adaptive immunity (signal 1) and augment antigen (signal 2)-induced allergic airway inflammation. To test whether inhibiting signal 1 by administering antioxidants inhibits allergic airway inflammation
E Duncanson et al.
Molecular & general genetics : MGG, 236(2-3), 275-282 (1993-01-01)
Eleven green individuals were isolated when 95000 M2 plants of barley (Hordeum vulgare L.), mutagenised with azide in the M1, were screened for nitrite accumulation in their leaves after nitrate treatment in the light. The selected plants were maintained in

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