17145

Clostridium difficile Agar (Base)

suitable for microbiology, NutriSelect^® Plus

• UNSPSC Code: 41171609
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15 g/L ; disodium phosphate, 5 g/L ; fructose, 6 g/L ; magnesium sulfate, 0.1 g/L ; monopotassium phosphate, 1 g/L ; proteose peptone, 40 g/L ; sodium chloride, 2 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 7.4±0.2 (25 °C)
• application(s): clinical testing; environmental; food and beverages; microbiology
• suitability: selective for Clostridium spp.

Description

Application

Used with supplement for cultivation of Clostridium difficile from food and certain pathological specimens.

Preparation Note

Suspend 34.55 g in 500 ml of distilled water. Heat gently to boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C. Aseptically add rehydrated contents of 1 vial of Clostridium Difficile Supplement (Cat. No. 17122) together with 7% (v/v) defibrinated Horse blood or Sheep blood. Mix well and pour into sterile petri plates.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves