MABE1900

Anti-AKNA Antibody, clone 4F5

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4F5, monoclonal
• mol wt: calculated mol wt 155.14 kDa; observed mol wt ~155 kDa
• purified by: using protein G
• species reactivity: human
• packaging: antibody small pack of 100 μL

Description

General description

Microtubule organization protein AKNA (UniProt: Q7Z591; also known as AT-hook-containing transcription factor, AKNA) is encoded by the AKNA (also known as KIAA1968) gene (Gene ID: 80709) in human. AKNA is a centrosomal protein that plays a key role in cell delamination by regulating microtubule organization. It is predominantly expressed by lymphoid tissues and its higher expression is observed in the spleen, lymph nodes and peripheral blood leukocytes. Its expression is reported in B- and T-lymphocytes, Natural killer cells and CD1a+CD14- but not CD1a-CD14+ dendritic cells. AKNA localizes to the distal part of the subdistal appendages of the mother centriole in interphase and is also found at the proximal ends of centrioles and along microtubules. It dissociates from centrosomes during M-phase and reassembles at the centrosomes during late telophase and early G1 phase. This dissociation and reassembly are regulated by its phosphorylation. AKNA presence is also reported in subdistal appendages of the mother centriole in specific subtypes of neural stem cells and all basal progenitors and is required for the delamination and retention of neural stem cells from the subventricular zone during neurogenesis. Eight different isoforms of AKNA have been described that are produced by alternative splicing. (Ref.: Ortega, GC., et al. (2019) Nature 567(7746); 113-117).

Specificity

Clone 4F5 is a rat monoclonal antibody that detects Microtubule organization protein AKNA. It targets an epitope within 11 amino acids from the C-terminal half.

Immunogen

Ovalbumin-conjugated linear peptide corresponding to 11 amino acids from the C-terminal half of human Microtubule organization protein AKNA.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected AKNA in HeLa cell lysate.

Tested Applications

Immunofluorescence Analysis: A representative lot detected AKNA in Immunofluorescence applications (Camargo Ortega, G., et al. (2019). Nature. 567(7746):113-117).

ELISA Analysis: A representative lot detected AKNA in ELISA applications (Camargo Ortega, G., et al. (2019). Nature. 567(7746):113-117).

Western Blotting Analysis: A representative lot detected AKNA in Western Blotting applications (Camargo Ortega, G., et al. (2019). Nature. 567(7746):113-117).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-AKNA, clone 4F5, Cat. No. MABE1900, is a rat monoclonal antibody that detects AKNA and is tested for use in ELISA, Immunofluorescence, and Western Blotting.

Physical form

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1