Please see the composition for each of the 3 buffers included in this kit:
Ni-NTA Bind Buffer: 50 mM NaH2PO4, pH 8.0; 300 mM NaCl; 10 mM imidazole
Ni-NTA Wash Buffer: 50 mM NaH2PO4, pH 8.0; 300 mM NaCl; 20 mM imidazole
Ni-NTA Elute Buffer: 50 mM NaH2PO4, pH 8.0; 300 mM NaCl; 250 mM imidazole
70899
Ni-NTA Buffer Kit
Synonym(s):
Buffer Kit for Ni-NTA, Ni-NTA Buffer
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About This Item
UNSPSC Code:
41106500
NACRES:
NA.56
Pricing and availability is not currently available.
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manufacturer/tradename
Novagen®
Quality Level
storage condition
OK to freeze
shipped in
ambient
storage temp.
10-30°C
General description
The Ni-NTA Buffer Kit provides a convenient set of buffers optimized for purification of His•Tag fusion proteins on Ni-NTA His•Bind Resin. These phosphate-buffered solutions differ from the Tris-based solutions used in the His•Bind Buffer Kit. Carefully prepared 4X concentrates are included for binding, washing and elution according to recommended protocols.
Components
•2 × 125 ml4X Ni-NTA Bind Buffer
•125 ml4X Ni-NTA Wash Buffer
•50 ml4X Ni-NTA Elute Buffer
•125 ml4X Ni-NTA Wash Buffer
•50 ml4X Ni-NTA Elute Buffer
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Hi I want to buy this kit: 70899 Ni-NTA Buffer Kit and want to also know the buffer composition. Since I am doing native protein purification its important for me to know the composition of the buffers. Please inform where I can find it. Thanks
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