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Key Documents

G005389

HPLC Analysis of an Energy Drink on Ascentis® Express RP-Amide (UV detection)

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 5 cm x 3 mm I.D., 2.7 μm particles (53916-U)
mobile phase: [A] water with 0.1% TFA; [B] acetonitrile with 0.1% TFA
flow rate: 0.6 mL/min
pressure: 1146 psi (79 bar)
column temp.: 35 °C
detector: UV, 254 nm
injection: 2 μL
sample: Energy Drink Diluted 1:9 in acetonitrile

suitability

application for HPLC

application(s)

food and beverages

Analysis Note

This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of common drink additives used in caffeinated energy drinks.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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