PP0100

Trypsin Profile IGD Kit

• Synonym(s): In gel digestion kit, Proteomics grade Trypsin, TPCK treated
• NACRES: NA.56
• UNSPSC Code: 12352200

Properties

• usage: kit sufficient for ≤100 applications
• Quality Level: 200
• storage temp.: 2-8°C

Description

General description

The Trypsin Profile IGD Kit enables fast, efficient, and complete in-gel tryptic digestion of up to 100 excised protein spots. Digested proteins are ready for MALDI-MS (matrix-assisted laser desorption/ionization-mass spectrometry) and require no additional preparation. Because the Trypsin Profile IGD Kit contains Proteomics Grade Trypsin, a higher sequence coverage and fewer ambiguous autolytic peaks are observed in MALDI spectra.
Trypsin has been chemically modified through reductive methylation of the ε-amino groups of lysine to reduce autolysis and minimize autolytic fragments. In addition, it has been TPCK (tosyl phenylalanyl chloromethyl ketone) treated to remove residual chymotrypsin activity and then further purified by affinity chromatography, yielding a highly purified trypsin suitable for proteomics work.

Application

Trypsin Profile IGD Kit has been used for in-gel digestion for LC-MS (liquid chromatography-mass spectrometry)/MS and MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry analysis.[1]

Features and Benefits

• Faster destaining than with alternative in-gel digest kits
• Includes 100% of the reagents needed to destain, digest, and extract proteins/peptides of interest
• Compatible with Coomassie, SYPRO^™ Orange, SYPRO Ruby, and ProteoSilver^™ Stained gels
• Resulting samples are ready for analysis by MALDI-MS or HPLC-MS

Legal Information

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC

SYPRO is a trademark of Thermo-Fisher Scientific, Inc.

Kit Components Only

• Destaining Solution Reconstituted to 75

Safety Information

• pictograms: GHS02,GHS08,GHS07
• signalword: Danger
• hcodes: H225,H302 + H312 + H332,H315,H319,H334,H335
• pcodes: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 3 - Flammable liquids
• flash_point_f: 35.6 °F - closed cup
• flash_point_c: 2.0 °C - closed cup
• wgk: WGK 2