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G7041

Sigma-Aldrich

Fish Gelatin

Gelatin from cold water fish skin
1 of 2 reviewers received a sample product or took part in a promotion

from cold water fish skin, powder, suitable for cell culture and generation of scaffolds.

Synonym(s):

Gelatine, Teleostean gelatin

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.75
Pricing and availability is not currently available.

Product Name

Gelatin from cold water fish skin, solid

biological source

fish skin

Quality Level

product line

BioReagent

form

solid

mol wt

60 kDa

packaging

pkg of 100 g
pkg of 500 g

technique(s)

cell culture | mammalian: suitable

surface coverage

100—200 μg/cm2

impurities

≤2% Residue on ignition (Ash)
≤20 ppm Heavy metals

solubility

acetic acid: soluble
glycerol: soluble
water: soluble

shipped in

ambient

storage temp.

room temp

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General description

Gelatin derived from cold water fish skin has low gelling and melting points.[1] This gelatin will not gel at 10 degree celsius, hence they do not have bloom strength.[2]

Application

Gelatin from cold water fish skin has been used in the preparation of water phase.[3] It has also been used to maintain growth in static culture[4][5] and in flavopiridol treatment and transcript labeling.[6]
Used as a blocking agent in immunochemistry.

Biochem/physiol Actions

Gelatin from fish skin can be used in the preparation of various gels based on their gelling characteristics. It can also be used as an additive in surimi processing to enhance the functional and mechanical properties of gel.[7]

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Certificates of Analysis (COA)

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Using fish gelatin and pectin to make a low-fat spread.
Cheng L H, et al.
Food Hydrocolloids, 22(8), 1637-1640 (2008)
Encapsulation and Controlled Release Technologies in Food Systems (2016)
Maximising the Value of Marine By-Products (2006)
Marine Proteins and Peptides: Biological Activities and Applications (2013)
Samia Rourou et al.
Applied microbiology and biotechnology, 85(1), 53-63 (2009-06-13)
Vero cells growth and rabies production in IPT-AF medium, a property animal-component-free medium are described in this work. Kinetics of cell growth and rabies virus (strain LP 2061) production were first conducted in spinner flasks. Over eight independent experiments, Vero

Questions

1–10 of 13 Questions  
  1. Hi, could you please let me know the fish species or the Amino Acid sequence of the gelatin? Thanks!

    1 answer
    1. Unfortunately, the source species of cold-water fish for this product is not available. No sequence information is available either.

      Helpful?

  2. What fish species is this gelatin product derived from? Thanks.

    1 answer
    1. This gelatin product is produced from multiple cold water fish species. These include, but are not limited to, cod, pollock, and haddock.

      Helpful?

  3. Is product G7041 acid pretreated or alkali pretreated?

    1 answer
    1. This product is extracted from acid-cured tissue.

      Helpful?

  4. Is this in solid powder form? Also, is it suitable to be used to form a hydrogel film?

    1 answer
    1. Yes, this product is supplied in solid powder form. Gelatin, in general, can be used to form a hydrogel film, but this product has not been tested for that application.

      Helpful?

  5. What is the molecular weight of this Gelatin?

    1 answer
    1. The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
      Bloom Number Average Molecular Mass (Da)
      50−125 (Low Bloom) 20,000−25,000
      175−225 (Medium Bloom) 40,000−50,000
      225−325 (High Bloom) 50,000−100,000

      Please see the link below to review the general datasheet for Gelatin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdf

      Helpful?

  6. Could you provide me with a preparation protocol to use it as a blocking agent in ELISA for G7041 ? And at what concentration is G7041 100 g.?

    1 answer
    1. This powdered gelatin product is not specifically tested for use as a blocking agent in Elisa. Typically, Fish Skin Gelatin is prepared in a buffer at a concentration of 1 - 5% for this application. Please see the link below to review an article that may be helpful.
      https://pubmed.ncbi.nlm.nih.gov/3611792/

      Please see the link below to review the general Gelatin datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/110/548/g7041pis.pdf

      Helpful?

  7. I would like to know the primary amine and functionable carboxylic acid group contents of this product.

    1 answer
    1. The primary amine and functionable carboxylic acid group contents of G7041 were not determined in-house.

      Helpful?

  8. How to store the product.

    1 answer
    1. As stated in the PROPERTIES section, this item is stored at room temperature. Please see the link below to review the product datasheet for additional information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/110/548/g7041pis.pdf

      Helpful?

  9. How long fish gelatin(g7041) is stable from the moment it dissolves?

    1 answer
    1. Sterile solutions of gelatin are quite stable when stored refrigerated. The 40 - 50% Solution of this product, G7765, is not assigned an expiration date, but it is retested every 2 years if stock remains. Please see the links below to review the general datasheet for gelatin products:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/187/614/g6650-et-al-pis-ms.pdf

      Please see the link below to review a sample Certificate of Analysis for the prepared Fish Gelatin solution:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/G7765-1LSLCQ7056.pdf

      Helpful?

  10. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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1–10 of 13 Questions  

Reviews

1 of 2 reviewers received a sample product or took part in a promotion

Active Filters

    • Review 1
    • Votes 4
    5 out of 5 stars.

    World's finest gelatin from cold water fish skin

    This is quite possibly the most exquisite gelatin from cold water fish skin I have ever used. Upon opening the bottle, I could tell just by the sheen on the slightly granular crystals that this gelatin from cold water fish skin was unlike any I had ever encountered.

    Helpful?

  1. 1 Ratings-Only Review

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