Yes, Ganciclovir G2536 can be dissolved in DMSO for in vitro application. Additionally, Ganciclovir is available in SML2346 as a ready-made DMSO solution, which is directly applicable for in vitro application.
G2536
Ganciclovir
≥99% (HPLC), powder, viral DNA elongation inhibitor
Synonym(s):
2-Amino-1,9-dihydro-9-[[2-hydroxy-1-(hydroxymethyl)ethoxy]methyl]-6H-purin-6-one, 9-(1,3-Dihydroxy-2-propoxymethyl)guanine, DHPG
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About This Item
Empirical Formula (Hill Notation):
C9H13N5O4
CAS Number:
Molecular Weight:
255.23
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.77
Pricing and availability is not currently available.
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Product Name
Ganciclovir, ≥99% (HPLC), powder
Quality Level
Assay
≥99% (HPLC)
form
powder
color
white
solubility
0.1 M HCl: 10 mg/mL
ε (extinction coefficient)
12.0 at 256 nm at 1 mM
antibiotic activity spectrum
viruses
Mode of action
DNA synthesis | interferes
originator
Roche
storage temp.
2-8°C
SMILES string
NC1=Nc2c(ncn2COC(CO)CO)C(=O)N1
InChI
1S/C9H13N5O4/c10-9-12-7-6(8(17)13-9)11-3-14(7)4-18-5(1-15)2-16/h3,5,15-16H,1-2,4H2,(H3,10,12,13,17)
InChI key
IRSCQMHQWWYFCW-UHFFFAOYSA-N
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General description
Chemical structure: nucleoside
Application
Ganciclovir is used in molecular biology for selection against random recombination events when homologous recombination of a gene of interest is required.
Biochem/physiol Actions
Ganciclovir is a pro-drug nucleoside analog that is activated by phosphorylation. It is useful in the study of gene therapy in cancer research.
Upon expression of a viral suicide gene encoding thymidine kinase, the non-toxic pro-drug is converted to a phosphorylated active analog and is incorporated into the DNA of replicating eukaryotic cells, causing death of the malignant dividing cell.[1] The cell cycle is irreversibly arrested at the G2-M checkpoint.[2] Gap junction involvement in the ganciclovir bystander effect has been studied.[3] Ganciclovir has been used to study loss of telomeres[4] and to evaluate sensitivity of viruses to antiviral treatments.[5]
Upon expression of a viral suicide gene encoding thymidine kinase, the non-toxic pro-drug is converted to a phosphorylated active analog and is incorporated into the DNA of replicating eukaryotic cells, causing death of the malignant dividing cell.[1] The cell cycle is irreversibly arrested at the G2-M checkpoint.[2] Gap junction involvement in the ganciclovir bystander effect has been studied.[3] Ganciclovir has been used to study loss of telomeres[4] and to evaluate sensitivity of viruses to antiviral treatments.[5]
Features and Benefits
This compound is a featured product for ADME Tox research. Click here to discover more featured ADME Tox products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
This compound was developed by Roche. To browse the list of other pharma-developed compounds and Approved Drugs/Drug Candidates, click here.
Preparation Note
Ganciclovir is soluble in 0.1 M HCl (10 mg/ml), DMSO (5 mg/ml), water (2 mg/ml), hot methanol, and ethanol (<1 mg/ml).
This product should be stored desiccated at 2-8 °C. Under these conditions the product is stable for 3 years.
This product should be stored desiccated at 2-8 °C. Under these conditions the product is stable for 3 years.
Ganciclovir is tested for solubility in 0.1M HCl at 10 mg/mL. The compound is also soluble in DMSO at 5 mg/mL.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Muta. 1B - Repr. 2
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Customers Also Viewed
Sunwen Chou
Reviews in medical virology, 18(4), 233-246 (2008-04-03)
Mutations in the human CMV UL97 kinase gene are a major mechanism of viral resistance to two anti-CMV drugs, ganciclovir (GCV) and maribavir (MBV). GCV, the most widely used and established therapy for CMV, is a substrate for the UL97
Zhanguo Gao et al.
Biomolecules, 11(10) (2021-10-24)
Duchenne muscular dystrophy (DMD), caused by the loss of dystrophin, remains incurable. Reduction in muscle regeneration with DMD is associated with the accumulation of fibroadipogenic progenitors (FAPs) differentiating into myofibroblasts and leading to a buildup of the collagenous tissue aggravating
C N Sprung et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(12), 6781-6786 (1999-06-09)
The addition of new telomeres to the ends of broken chromosomes, termed chromosome healing, has been extensively studied in unicellular organisms; however, its role in the mammalian cell response to double-strand breaks is unknown. A system for analysis of chromosome
Julie Cates Scott et al.
Therapeutic drug monitoring, 26(1), 68-77 (2004-01-30)
The authors use a previously published decision-making algorithm to address the role of clinical pharmacokinetic monitoring of ganciclovir, the drug of choice for prophylaxis and treatment of cytomegalovirus (CMV) in solid organ transplant recipients. Ganciclovir pharmacokinetics have been studied in
L Z Rubsam et al.
Cancer research, 59(3), 669-675 (1999-02-11)
The ability of herpes simplex virus type 1 thymidine kinase (HSV-TK)-expressing cells incubated with ganciclovir (GCV) to induce cytotoxicity in neighboring HSV-TK-negative (bystander) cells has been well documented. Although it has been suggested that this bystander cell killing occurs through
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Can Ganciclovir G2536 be dissolved in DMSO for in vitro testing?
1 answer-
Helpful?
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can ganciclovir be dissolved in PBS or saline? can ganciclovir be neutralized by 0.1M NaOH after dissolving in 0.1M HCl
1 answer-
Product G2536, Ganciclovir is tested for solubility in 0.1M HCl at 10 mg/mL. The compound is also soluble in DMSO at 5 mg/mL. While pH adjustment for neutralization has not been tested, this is a typical practice in pharmaceutical-grade solutions for injection. It is anticipated that no issues would arise regarding stability or precipitation.
See below for a link to a typical product insert, page 14 under Description:
https://www.accessdata.fda.gov/drugsatfda_docs/label/2017/209347lbl.pdfHelpful?
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Can ganciclovir be resuspended in solutions other than HCl? If so, what is the solubility and storage condition? Thank you.
1 answer-
References state that this chemical is soluble in 0.1 M HCl (10 mg/ml), DMSO (5 mg/ml), water (2 mg/ml), hot methanol, and ethanol (<1 mg/ml).
This product should be stored desiccated at 2-8 °C. Under these conditions the product is stable for 3 years.
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