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F5554

Sigma-Aldrich

Formalin, 10%, Neutral Buffered, Wintergreen Scented

histological fixative, pH 6.9-7.1

Synonym(s):

Formaldehyde, Methanal

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.47

Quality Level

shelf life

Expiry date on the label.

color

colorless

pH

6.90-7.10

application(s)

hematology
histology

storage temp.

room temp

General description

10% Neutral buffered formalin (NBF) is the most commonly used fixative for light microscopy. 10% NBF is well suited for large throughput laboratories and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship.

Application

Formalin, 10%, Neutral Buffered, Wintergreen Scented has been used as a fixative in transformation and tumorigenicity assay and in immunohistostaining.
Intended for use as a general purpose histological fixative. Wintergreen oil has been added to the standard formulation to provide a wintergreen scent.

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Effects of formalin fixation on tissue optical
M F G Wood
Physics in Medicine and Biology, 56, V115-V115 (2011)
Toward deciphering proteomes of formalin-fixed paraffin-embedded (FFPE) tissues
Magdeldin, et al.
Proteomics, 12(7), 1045-1058 (2012)
A method to estimate the formaldehyde concentration in fixative solutions
Simos, P
Pathology, 43, 394-394 (2001)
Flow cytometric determination of stem/progenitor content in epithelial tissues: an example from nonsmall lung cancer and normal lung
Donnenberg VS, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 83(1), 141-149 (2013)
Kelly B Engel et al.
Archives of pathology & laboratory medicine, 135(5), 537-543 (2011-04-30)
While formalin fixation and paraffin embedding has become a universal mechanism of tissue preservation and a gold standard for immunohistochemistry, fixation and processing variables that may confound assay effectiveness have received little attention from the scientific community. To identify discrete

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