700 mm Hg (27.5” Hg, 0.9 bar) differential vacuum at 25 °C (77 °F)
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About This Item
Quality Segment
material
polyethersulfone membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing
product line
Stericup®
feature
hydrophilic
packaging
pack of 12 ea
greener alternative product characteristics
Waste Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
parameter
max. temp. at 45 °C
description
2.4 mL (hold-up volume)
technique(s)
sterile filtration: suitable
H
193 mm
diam.
9.3 cm
filter capacity
500 mL
filter diam.
73 mm
filtration area
40 cm2
matrix
Express PLUS™
pore size
0.22 μm pore size
fitting
inlet thread(s), 38 mm (1 1/2 in.) thread
greener alternative category
shipped in
ambient
storage temp.
no temp limit
General description
The Stericup® E and Steritop® E sterile filters are designed as a Greener Alternative for vacuum sterile filtration that significantly reduces plastic and packaging.
This Stericup® E is a 38mm thread collar which uses a 0.22 μm pore size polyethersulfone (PES) membrane filter unit combined with a 500 mL receiver flask and cap for processing and storage. The 38 mm thread directly connects to our media bottles and majority of other standard commercial media bottles.
Use only glass or plastic bottles designed for vacuum applications.
Application
- Tissue culture media +/- additives
- Buffers
- Biological solutions
Features and Benefits
- The filter collar disconnects from the receiver bottle with just a quarter turn—reducing the likelihood of spillage and making it easier to manipulate on the bench or under the hood.
- Bold product identification on the filter collar plainly displays membrane characteristics, including composition and pore size, for rapid, application-appropriate product selection.
- Click seal cap is flanged and modified to enable a firm grip in wet or dry conditions—and a tactile stop confirms secure closure. Worry less about the possibility of spilling or contamination of sterilized contents.
- Frosted, ample writing surface on the receiver bottle and lighter cap color facilitate clear labelling to improve legibility and reduce unreadable smears.
- Click seal cap rests on the side to avoid risk of contamination.
- Receiver bottles are stackable to save storage space.
Legal Information
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| description pore size 0.22 μm, polyethersulfone membrane, filter capacity 500 mL, 38 mm (1 1/2 in.) thread, pack of 12 ea | description pore size 0.22 μm, polyethersulfone membrane, capacity 500 mL, 45 mm thread, pack of 12 ea | description pore size 0.22 μm, polyethersulfone membrane, filter capacity 1000 mL, 38 mm (1 1/2 in.) thread, pack of 12 ea | description pore size 0.22 μm, polyethersulfone membrane, filter capacity 1000 mL, 45 mm thread, pack of 12 ea |
| pore size 0.22 μm pore size | pore size 0.22 μm pore size | pore size 0.22 μm pore size | pore size 0.22 μm pore size |
| filter capacity 500 mL | filter capacity 500 mL | filter capacity 1000 mL | filter capacity 1000 mL |
| material polystyrene funnel(s), polyethersulfone membrane, polystyrene bottle, polystyrene housing | material polystyrene bottle, polystyrene housing, polyethersulfone membrane, polystyrene funnel(s) | material polyethersulfone membrane, polystyrene funnel(s), polystyrene bottle, polystyrene housing | material polystyrene funnel(s), polystyrene housing, polyethersulfone membrane, polystyrene bottle |
| feature hydrophilic | feature hydrophilic | feature hydrophilic | feature hydrophilic |
| packaging pack of 12 ea | packaging pack of 12 ea | packaging pack of 12 ea | packaging pack of 12 ea |
| fitting 38 mm (1 1/2 in.) thread, inlet thread(s) | fitting inlet thread(s), 45 mm thread | fitting inlet thread(s), 38 mm (1 1/2 in.) thread | fitting inlet thread(s), 45 mm thread |
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What is the maximum vacuum pressure for filtration?
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What is the maximum processing volume for Stericup® and Steritop® filters? Can I add more volume after initial filtration?
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All Stericup® and Steritop® filters were developed for single-use application. The maximum volume is dependent on the receiver bottle capacity. Stericup® filters come with a receiver bottle with a specified maximum volume. For Steritop® filters, the maximum recommended volume is 2 L with an appropriate-sized receiver bottle. Larger processing volumes can result in membrane fouling, increase chance of spillage and contamination, and lead to possible airlock*.
*Note that devices with PES membranes can airlock if filtered to dryness. Some liquid must be left on top of the filter, then the system stopped, receiver changed or emptied, and more sample volume added before restart. This increases the chance of spillage.Helpful?
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Can Stericup® bottles be placed inside freezers for long-term storage of media?
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You can successfully freeze and store many aqueous solutions (such as culture media) in Stericup® bottles at temperatures up to -20 °C (-4 °F). It is strongly recommended that you run a sample stability trial under your actual storage conditions prior to using Stericup® bottles for frozen storage.
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Can Stericup® and Steritop® filters be reused?
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All Stericup® and Steritop® filters were developed for single use, disposable processing.
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What is the expiration date of Stericup® and Steritop® filters?
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Typically 3 years from sterilization.
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Are Stericup® and Steritop® filters GMP-certified?
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No, Stericup® and Steritop® devices are not GMP-approved.
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Can Stericup® and Steritop® filters be used to prepare solutions that will be administered to patients?
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No, all Stericup® and Steritop® devices are Research Use Only.
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What Bacterial Endotoxins Test is used?
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The test method used complies with USP 85 and EP 2.6.14 to ensure the absence of endotoxin in our products.
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What tubing can be used with Stericup® and Steritop® vacuum filters?
What tubing can be used with Stericup® and Steritop® vacuum filters?
1 answer-
Stericup® and Steritop® filters are fitted with a 1/4” tube adapter. We recommend 3/16 in. (4.8 mm) ID silicone tubing to fit this tube adapter: https://www.sigmaaldrich.com/product/mm/xx7100004
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What leachables are detected during filtration processes using Stericup® filters?
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We have not performed extractables/leachables studies for our Stericup® filters.
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