444805
Membrane Traffic Inhibitor, A5
The Membrane Traffic Inhibitor, A5 controls the biological activity of adaptor complex AP-1.
Synonym(s):
Membrane Traffic Inhibitor, A5, 1-(4-(4-(E-2-Methyl-2-butenyl)piperazinyl)phenyl)ethanone, HCl, 5431942, A5
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About This Item
Empirical Formula (Hill Notation):
C17H24N2O · xHCl
Molecular Weight:
272.39 (free base basis)
UNSPSC Code:
12352200
NACRES:
NA.77
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Quality Level
assay
≥97% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
protect from light
color
pale yellow
solubility
water: 1 mg/mL
shipped in
ambient
storage temp.
−20°C
General description
A cell-permeable piperazinyl compound that specifically blocks clathrin adaptor complex AP-1-dependent traffic between trans-Golgi network (TGN) and endosomes in budding yeast, while exhibiting little effects against other trafficking pathways involving TGN and endosomes. Inhibits growth of yeast cells lacking complementary GGA1/2 adaptor proteins (EC50 = 50 µM), but not wild-type or AP-1-deficient cells even at concentrations as high as 300 µM. A5 presumably acts at a stage after AP-1 membrane recruitment as evidenced by an enhanced AP-1 perinuclear localization upon A5 treatment of HeLa cells.
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Duncan, M.C., et al. 2007. Proc. Natl. Acad. Sci. USA104, 6235.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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