Skip to Content
MilliporeSigma
All Photos(1)

Documents

434631

Sigma-Aldrich

Anti-Lewis x Mouse mAb (P12)

liquid, clone P12, Calbiochem®

Synonym(s):

Anti-Le x, Anti-Lex

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

tissue culture supernatant

antibody product type

primary antibodies

clone

P12, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgM

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FUT3(2525)

General description

Purified mouse monoclonal antibody. Recognizes the Lewis x type 2 oligosaccharide chain.
Recognizes the Lewis x carbohydrate chain in normal kidney.
This Anti-Lewis x Mouse mAb (P12) is validated for use in Paraffin Sections for the detection of Lewis x.

Immunogen

Human
fresh (non-cultured) human placenta cells

Application

Paraffin Sections (1:10-1:120)

Warning

Toxicity: Standard Handling (A)

Physical form

Tissue Culture Supernatant

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Normal kidney tissue

Other Notes

For paraffin sections, the recommended working dilution is ≥1:40 (with biotin based detection systems). For fixation, 10% formalin buffered to neutral pH is recommended. Variables associated with assay conditions will dictate the proper working dilutions.
Ree, H.J., et al. 1991. Cancer67, 1338.
Cordon-Cardo, C., et al. 1986. Int. J. Cancer37, 667.
Sakamoto, J., et al. 1986. Cancer Res. 46, 1553
Sakamoto, J., et al. 1984. Mol. Immunol. 21, 1093.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Ernesto Rodriguez et al.
Communications biology, 5(1), 41-41 (2022-01-13)
Pancreatic ductal adenocarcinoma (PDAC) remains one of the most aggressive malignancies with a 5-year survival rate of only 9%. Despite the fact that changes in glycosylation patterns during tumour progression have been reported, no systematic approach has been conducted to
Chen Lu et al.
Journal of visualized experiments : JoVE, (63), e3791-e3791 (2012-05-17)
In this study, we describe an effective protocol for use in a multiplexed high-throughput antibody microarray with glycan binding protein detection that allows for the glycosylation profiling of specific proteins. Glycosylation of proteins is the most prevalent post-translational modification found
Sandy R Pernitzsch et al.
Nature communications, 12(1), 4433-4433 (2021-07-23)
The small, regulatory RNA RepG (Regulator of polymeric G-repeats) regulates the expression of the chemotaxis receptor TlpB in Helicobacter pylori by targeting a variable G-repeat in the tlpB mRNA leader. Here, we show that RepG additionally controls lipopolysaccharide (LPS) phase

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service