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119102

Sigma-Aldrich

Anti-H+,K+-ATPase, α-Subunit (1-17) Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

antibody product type

primary antibodies

clone

polyclonal

form

liquid

species reactivity

canine, mouse, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

General description

This product has been discontinued.



Recognizes the α subunit of H+,K+ ATPase.

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the α subunit of H+-K+ ATPase.

Immunogen

Porcine
a synthetic peptide (MGKAENYELYQVELGPGC) corresponding to amino acids 1-17 of porcine H+, K+-ATPase α-subunit

Application

ELISA (see comments)

Immunoblotting (1:1000)

Immunocytochemistry (see comments)

Paraffin Sections (see comments)

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following initial thaw, aliquot and freeze (-70°C). Recommended dilution buffer is Tris buffered saline with 1% BSA, pH 7.5.

Other Notes

Callaghan, J.M., et al. 1995. Am. J. Physiol. 268, F363.
Smolka, A. 1992. Biophys. Biochem. Acta 1108, 75.
Does not cross-react with Na+, K+-ATPase. This antibody is also reported to work for ELISA, immunocytochemistry, and paraffin sections.Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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With the aims of sharing information about the technical aspects of immunohistochemistry (IHC) and making it possible to make a suitable choice of antibody for histopathological examination, this technical report describes the results of a questionnaire administered during the period

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