HPLC Analysis of Adenosine Nucleosides on Supelcosil^™ LC-18-T

column

SUPELCOSIL LC-18-T, 15 cm x 4.6 mm I.D, 3 μm particles (58970-U)

mobile phase

[A] 0.1 M potassium phosphate, pH 7; [B] acetonitrile

gradient

0 to 2.5% B in 4 min, to 7.5% B in 2 min, to 0% B in 0.1 min, held at 0% B for 3.9 min

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 254 nm

injection

20 μL

sample

each compound 5 μg/mL in water