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X4126

Xylene Cyanol FF

BioReagent, Molecular Biology

Synonym(s):

Acid blue 147, Cyanol FF, XC

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Pack SizeSKUAvailabilityPrice
10 g

Estimated to ship TODAYfromMILWAUKEE

$128.00

About This Item

Empirical Formula (Hill Notation):
C25H27N2NaO6S2
CAS Number:
2650-17-1
Molecular Weight:
538.61
UNSPSC Code:
12171500
NACRES:
NA.52
PubChem Substance ID:
57654732
EC Number:
220-167-5
Colour Index Number:
42135
MDL number:
MFCD00019481

$128.00

Estimated to ship TODAYDetails

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grade

Molecular Biology

Quality Level

200

product line

BioReagent

form

powder

technique(s)

nucleic acid detection: suitable

solubility

H2O: complete 1 mg/mL, clear, blue

suitability

suitable for nucleic acid staining

storage temp.

room temp

SMILES string

[Na+].CCNc1ccc(cc1C)C(=C2/C=CC(=N\CC)\C(C)=C2)\c3ccc(cc3S(O)(=O)=O)S([O-])(=O)=O

InChI

1S/C25H28N2O6S2.Na/c1-5-26-22-11-7-18(13-16(22)3)25(19-8-12-23(27-6-2)17(4)14-19)21-10-9-20(34(28,29)30)15-24(21)35(31,32)33;/h7-15,26H,5-6H2,1-4H3,(H,28,29,30)(H,31,32,33);/q;+1/p-1/b25-19-,27-23-;

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This Item
B3269335940205486
Xylene Cyanol FF BioReagent, Molecular Biology

Sigma-Aldrich

X4126

Xylene Cyanol FF

Bromphenol Blue–Xylene Cyanole Dye solution lyophilized powder

Sigma-Aldrich

B3269

Bromphenol Blue–Xylene Cyanole Dye solution

Xylene Cyanol FF Dye content ≥75 %

Sigma-Aldrich

335940

Xylene Cyanol FF

Xylenol Blue indicator grade, Dye content 90 %

Sigma-Aldrich

205486

Xylenol Blue

suitability

suitable for nucleic acid staining

suitability

-

suitability

-

suitability

-

grade

Molecular Biology

grade

-

grade

-

grade

indicator grade

technique(s)

nucleic acid detection: suitable

technique(s)

-

technique(s)

-

technique(s)

titration: suitable

form

powder

form

lyophilized powder

form

powder or crystals

form

powder

product line

BioReagent

product line

-

product line

-

product line

-

solubility

H2O: complete 1 mg/mL, clear, blue

solubility

water: 0.2 vial/mL, blue

solubility

water: 1 mg/mL, blue

solubility

-

General description

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

Xylene Cyanol FF is used as a:
  • histological stain and as tracker dye in DNA sequencing and electrophoresis[1][2]
  • gel-loading dye
  • formamide RNA loading dye
  • tracking dye in native polyacrylamide gel electrophoresis (PAGE) loading buffer

Biochem/physiol Actions

Xylene Cyanol FF acts as nucleic acid stain.

Preparation Note

A 6x Gel Loading Buffer can be made using standard recipes, including 0.05% xylene cyanol.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS3724
SHBR7866
SHBM1857
SHBL0446
MKCF5562

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Nicolás Cara et al.
Bio-protocol, 10(13), e3671-e3671 (2021-03-05)
Methylation-Sensitive Amplification Polymorphism (MSAP) is a versatile marker for analyzing DNA methylation patterns in non-model species. The implementation of this technique does not require a reference genome and makes it possible to determine the methylation status of hundreds of anonymous
Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism
<BIG>Ota M, et al.</BIG>
Nature Protocols, 2857-2857 (2007)
P N Hengen
Trends in biochemical sciences, 20(5), 202-203 (1995-05-01)
Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methods-reagents, available on the Internet. This month's column describes how to make an electrophoresis system from common household items. For details on how to partake
Design and self-assembly of siRNA-functionalized RNA nanoparticles for use in automated nanomedicine
<BIG>Afonin KA, et al.</BIG>
Nature Protocols, 6, 2022-2022 (2011)
Cell-internalization SELEX: method for identifying cell-internalizing RNA aptamers for delivering siRNAs to target cells
<BIG>Thiel WH, et al.</BIG>
Methods in Molecular Biology, 187-199 (2015)
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Global Trade Item Number

SKUGTIN
X4126-10G04061835509966

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