This product is deionized and distilled during production. However, it is not subjected to ICP analysis, so it is not warranted to be ion-free.
W3500
Water
sterile-filtered, BioReagent, suitable for cell culture
Synonym(s):
Cell culture grade water, Ultra-filtered Water
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 mL | Available to ship onMarch 24, 2026fromMILWAUKEE | $16.00 | |
| 500 mL | Check Cart for Availability | $22.00 | |
| 1 L | Available to ship onMarch 24, 2026fromMILWAUKEE | $44.40 | |
| 6 x 500 mL | Estimated to ship onMay 18, 2026fromMILWAUKEE | $125.00 | |
| 6 x 1 L | Check Cart for Availability | $229.00 | |
| 24 x 500 mL | Estimated to ship onMay 18, 2026fromMILWAUKEE | $459.00 |
About This Item
Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
EC Number:
231-791-2
Beilstein/REAXYS Number:
2050024
MDL number:
Technique(s):
cell culture | mammalian: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
vapor density
<1 (vs air)
Quality Level
vapor pressure
3 mmHg
sterility
sterile-filtered
product line
BioReagent
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/mL endotoxin
refractive index
n20/D 1.34 (lit.)
pH
5-7
Looking for similar products? Visit Product Comparison Guide
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) FISH: suitable |
| Quality Level 400 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form liquid | form liquid | form liquid | form liquid |
| impurities ≤1 EU/mL endotoxin | impurities - | impurities - | impurities DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected |
| vapor density <1 (vs air) | vapor density - | vapor density - | vapor density - |
| pH 5-7 | pH - | pH - | pH 5-7 |
General description
This product is a very low endotoxin (less than 1 EU/mL), 0.2 micron filtered.
Application
For use in the preparation of cell culture media, and cell suspension and washing solutions.
Other Notes
Easily compare specifications for Water products with the Water specification table
comparable product
Product No.
Description
Pricing
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Philippe Robert et al.
Investigative radiology, 50(8), 473-480 (2015-06-25)
To prospectively compare in healthy rats the effect of multiple injections of macrocyclic (gadoterate meglumine) and linear (gadodiamide) gadolinium-based contrast agents (GBCAs) on T1-weighted signal intensity in the deep cerebellar nuclei (DCN), including the dentate nucleus. Healthy rats (n =
Mary A Rodgers et al.
The Journal of experimental medicine, 211(7), 1333-1347 (2014-06-25)
Linear ubiquitination is a newly discovered posttranslational modification that is currently restricted to a small number of known protein substrates. The linear ubiquitination assembly complex (LUBAC), consisting of HOIL-1L, HOIP, and Sharpin, has been reported to activate NF-κB-mediated transcription in
Yasushi Itoh et al.
PLoS pathogens, 10(6), e1004192-e1004192 (2014-06-20)
Highly pathogenic avian influenza (HPAI) viruses of the H5N1 subtype often cause severe pneumonia and multiple organ failure in humans, with reported case fatality rates of more than 60%. To develop a clinical antibody therapy, we generated a human-mouse chimeric
Liming Zhou et al.
Nature, 509(7498), 86-90 (2014-04-25)
Tropical forests are global epicentres of biodiversity and important modulators of climate change, and are mainly constrained by rainfall patterns. The severe short-term droughts that occurred recently in Amazonia have drawn attention to the vulnerability of tropical forests to climatic
Katrin Linke et al.
Biochimica et biophysica acta, 1837(1), 14-32 (2013-08-28)
Water is clearly important for the functioning of Photosystem II (PSII). Apart from being the very substrate that needs to be transported in this water oxidation enzyme, water is also vital for the transport of protons to and from the
Articles
FAQs on bacterial endotoxin contamination, details on endotoxin testing using the LAL assay, and tips to avoid contamination in cell cultures.
High-quality ultrapure water that is endotoxin-free is recommended for mammalian cell culture as it support cell viability and prevents contamination.
Protocols
Preparation from Packaged Powder, Preparation from Basal Salt Solutions, Banana Powder Preparation and Use, Coconut Water Preparation and Use
Related Content
To prepare 1L of a 10 X iron chelate solution
Global Trade Item Number
| SKU | GTIN |
|---|---|
| W3500-100ML | 04061838447357 |
| W3500-24X500ML | 04061838260154 |
| W3500-500ML | 04061838447371 |
| W3500-6X500ML | 04061838260161 |
| W3500-1L-CO | 04061837036880 |
| W3500-1L | 04061838447364 |
| W3500-6X1L | 04061837536496 |
| W3500-2L | 04061837536472 |
-
Is this product deionized?
1 answer-
Helpful?
-
-
How is this product used?
1 answer-
This product is tested for use in cell culture. Parameters such as cytotoxicity, pH, and endotoxin are measured. It may be used for any general application but is of most interest to customers doing cell culture work. It has not been tested for RNAse, DNAse, or protease activity.
Please see the link below to review a sample Certificate of Analysis:
https://www.sigmaaldrich.com/coa/SIGMA/W3500/RNBL9398Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service