SHP002
CRISPR & MISSION® Lentiviral Packaging Mix
Synonym(s):
Lentiviral Packaging Mix
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| Pack Size | SKU | Availability | Price |
|---|
About This Item
concentration
100 ng/μL (in Tris-EDTA)
Quality Level
application(s)
CRISPR
shipped in
dry ice
storage temp.
−20°C
Related Categories
1 of 1
This Item | |||
|---|---|---|---|
| concentration 100 ng/μL (in Tris-EDTA) | concentration - | concentration - | concentration ≥1x106 VP/ml (via p24 assay) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
| shipped in dry ice | shipped in - | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| application(s) CRISPR | application(s) - | application(s) - | application(s) CRISPR |
General description
Lentiviral particles are generated from three main components:
- The packaging vector, which contains the minimal set of lentiviral genes required to generate the virion structural proteins and packaging functions.
- The vesicular stomatitis virus G-protein envelope vector, which provides the heterologous envelope for pseudotyping and allows these lentiviral particles to efficiently deliver the transfer sequence of interest to a wide variety of cell types, including primary and non-dividing cells
- The lentiviral transfer vector, which contains the sequence of interest as well as the cis acting sequences necessary for packaging.
The Lentiviral Packaging Mix contains the first two components; it is designed to be co-transfected along with a compatible lentiviral transfer vector in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications. This product is recommended for packaging Lenti CRISPR, Lenti ORF, and Lenti shRNA vectors.
Application
Legal Information
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.
Protocols
FACS sorts cells based on light scattering and fluorescence for objective cell analysis.
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
Related Content
Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SHP002-0.25ML | 04061838043719 |
| SHP002-1.7ML | 04061838248848 |
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