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P7280

Sigma-Aldrich

Poly-ᴅ-Lysine Hydrobromide

synthetic, mol wt 30,000-70,000, powder, γ-irradiated, suitable for cell culture, BioXtra

Synonym(s):

PDL

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About This Item

Linear Formula:
D-Lys-(D-Lys)n-D-Lys · xHBr
CAS Number:
27964-99-4
MDL number:
MFCD00131934
UNSPSC Code:
12352202
PubChem Substance ID:
24898835
NACRES:
NA.75

product name

Poly-D-lysine hydrobromide, average mol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

biological source

synthetic (organic)

Quality Level

200

sterility

γ-irradiated

product line

BioReagent
BioXtra

form

lyophilized powder

mol wt

average mol wt 30,000-70,000

packaging

pkg of 5 X 5 mg
pkg of 5 mg

concentration

0.016—0.032 mmol lysine

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm2

solubility

H2O: soluble 50 mg/mL, clear, colorless

shipped in

ambient

storage temp.

−20°C

SMILES string

O=C(C)[C@@](NC)([H])CCCCN.[Br]

InChI

1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H

InChI key

MEXAGTSTSPYCEP-UHFFFAOYSA-N

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General description

Poly-D-Lysine Hydrobromide (PDL), a positively charged polymer consisting of lysine residues, where each lysine unit is associated with a hydrobromide molecule. This hydrobromide molecule gives the poly-D-lysine a unique characteristic, enabling it to form crystals and making it easily soluble in water-based solutions.

Application

Poly d lysine hydrobromide coating has been used:
  • In coating tissue culture plate wells and coverslips for immunofluorescence microscopy of primary neuronal culture to study neurological disorders associated with Zika virus infection.
  • To coat the coverslips for enhancing the attachment and proliferation of the myenteric cells isolated from the rat intestines.
  • In preparing surface of coverslips for cell attachment for studying the myenteric plexus of rat’s small intestine.
Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly-D-lysine (PDL) hydrobromide is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.

Features and Benefits

Sterilized using γ-irradiation

Components

Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the ß structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Preparation Note

This product has a molecular weight of 30,000-70,000 and is cell culture tested and gamma-irradiated. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Methods to Study the Myenteric Plexus of Rat Small Intestine
Hecking I, et al.
Cellular and Molecular Neurobiology, 43, 315?325-315?325 (2021)
Valentina Cigliola et al.
PloS one, 11(3), e0150880-e0150880 (2016-03-10)
Signalling through gap junctions contributes to control insulin secretion and, thus, blood glucose levels. Gap junctions of the insulin-producing β-cells are made of connexin 36 (Cx36), which is encoded by the GJD2 gene. Cx36-null mice feature alterations mimicking those observed
Progesterone: A Neuroprotective Steroid of the Intestine
Stegemann LN, et al.
Cells, 12(8), 1206-1206 (2023)
Nadine Dupuis et al.
Molecular pharmacology, 91(6), 595-608 (2017-03-21)
G protein-coupled receptors are the most important drug targets for human diseases. An important number of them remain devoid of confirmed ligands. GPR27 is one of these orphan receptors, characterized by a high level of conservation among vertebrates and a
Zika virus infection of mature neurons from immunocompetent mice generates a disease-associated microglia and a tauopathy-like phenotype in link with a delayed interferon beta response
Manet C, et al.
Journal of Neuroinflammation, 19 (2022)
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Protocols

Poly-L-Lysine Cell Attachment Protocol

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

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