MAK001
Alanine Assay Kit
sufficient for 100 colorimetric or fluorometric tests
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
usage
sufficient for 100 colorimetric or fluorometric tests
detection method
colorimetric, fluorometric
storage temp.
−20°C
General description
Alanine is a nonessential amino acid that plays a key role in the glucose-alanine cycle between muscle tissue and the liver. In amino acid-degrading tissues such as muscle, amino groups are pooled as glutamate by transamination reactions. The amino group of glutamate is transferred to pyruvate via alanine aminotransferase, forming alanine and α-ketoglutarate. The alanine is passed into the blood and transported to the liver. This reaction is reversed in the liver where pyruvate can be used in gluconeogenesis to form glucose, which may return to other tissues through the circulatory system. Increased alanine levels correlate with higher blood pressure, energy intake, cholesterol levels, and body mass index.
Application
Alanine Assay Kit has been used to determine the intracellular alanine pool.
Suitable for alanine detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.
Biochem/physiol Actions
In this kit, alanine concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the alanine present. Typical detection ranges for this kit are 2-10 nmole (colorimetric) and 0.2-1 nmole (fluorometric).
comparable product
Product No.
Description
Pricing
signalword
Danger
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
wgk
WGK 3
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
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Articles
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Protocols
Assay protocol for the colorimetric/fluorometric detection of Alanine in biological samples using the alanine assay kit.
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