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M7257

α-Mannosidase from Canavalia ensiformis (Jack bean)

ammonium sulfate suspension, ≥15 units/mg protein (biuret)

Synonym(s):

α-D-Mannoside mannohydrolase

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About This Item

CAS Number:
9025-42-7
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-794-1
MDL number:
MFCD00130495
EC Number:
3.2.1.24 (BRENDA, IUBMB)

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Product Name

α-Mannosidase from Canavalia ensiformis (Jack bean), ammonium sulfate suspension, ≥15 units/mg protein (biuret)

SMILES string

[Zn+2]

InChI key

PTFCDOFLOPIGGS-UHFFFAOYSA-N

InChI

1S/Zn/q+2

biological source

Canavalia ensiformis

form

ammonium sulfate suspension

specific activity

≥15 units/mg protein (biuret)

foreign activity

β-galactosidase, β-N-acetylglucosaminidase, α-galactosidase and α-L-fucosidase <0.05%

storage temp.

2-8°C

Quality Level

200

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This Item
A2264M940094281
&#945;-Mannosidase from Canavalia ensiformis (Jack bean) ammonium sulfate suspension, &#8805;15&#160;units/mg protein (biuret)

Sigma-Aldrich

M7257

α-Mannosidase from Canavalia ensiformis (Jack bean)

&#946;-N-Acetylglucosaminidase from Canavalia ensiformis (Jack bean) ammonium sulfate suspension, &#8805;10&#160;units/mg protein

Sigma-Aldrich

A2264

β-N-Acetylglucosaminidase from Canavalia ensiformis (Jack bean)

&#946;-Mannosidase from Helix pomatia 5-30&#160;units/mL, ammonium sulfate suspension, crude extract

Sigma-Aldrich

M9400

β-Mannosidase from Helix pomatia

Urease from Canavalia ensiformis (Jack bean) powder, white, ~8&#160;U/mg

Sigma-Aldrich

94281

Urease from Canavalia ensiformis (Jack bean)

specific activity

≥15 units/mg protein (biuret)

specific activity

≥10 units/mg protein

specific activity

-

specific activity

~8 U/mg

biological source

Canavalia ensiformis

biological source

Canavalia ensiformis

biological source

-

biological source

Canavalia ensiformis

form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

foreign activity

β-galactosidase, β-N-acetylglucosaminidase, α-galactosidase and α-L-fucosidase <0.05%

foreign activity

α- and β-galactosidase, and α-L-fucosidase ≤0.1%, α-mannosidase ≤0.2%

foreign activity

β-N-acetylglucosaminidase and α-mannosidase <1.0%

foreign activity

-

Biochem/physiol Actions

α-Mannosidase cleaves near α1−2-, α1−3- and α1−6-linked mannose residues.[1]
α-Mannosidase is an acid hydrolase which is located in plant vacuoles and is thought to be involved with the turnover of N-linked glycoproteins.
α−Mannosidase has been shown to inhibit the proliferation of B-lymphocytes. [2]

General description

α−Mannosidase from Canavalia ensiformis is a tretamer composed of two subunits that each contain two components at 44 and 66 kDa. [2]
α-Mannosidase is an exo-glycosidase and an inc metalloenzyme.[1]
Lysosomal α-mannosidases are class II mannosidases that belong to glycoside hydrolase family 38 and play an important role in the degradation of asparagine-linked carbohydrates of glycoproteins. [3]

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-D-mannoside to p-nitrophenol and D-mannose per min at pH 4.5 at 25°C.

Physical form

Suspension in 3.0 M (NH4)2SO4 and 0.1 mM zinc acetate, pH 7.5

Application

α-Mannosidase can be used to liberate mannose from a variety of synthetic and natural α-mannosides. It has also been used in a study to investigate the causes of neurodegeneration in mucolipidosis II ′knock-in′ mice. [4]
α-Mannosidase from Canavalia ensiformis (Jack bean) has been used:
  • for the digestion of neutral glycans.[5]
  • to remove O-glycans from recombinant β-glucosidase[6]
  • for the inhibition of renal fibroblasts proliferation[7]

Liberates mannose from a variety of synthetic and natural α-mannosides.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000477214
0000477213
0000477214
0000477213
0000443192

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Reto Buser et al.
The Journal of biological chemistry, 285(14), 10715-10723 (2010-01-12)
Coprinopsis cinerea is a model organism for fruiting body development in homobasidiomycetes. Here, we focused on N-linked oligosaccharides (NLO) of cell wall proteins in the hyphae of two developmental stages, vegetative mycelium and fruiting body. High mannose-type glycans were the
Engineering a highly active thermophilic beta-glucosidase to enhance its pH stability and saccharification performance
Xia W, et al.
Biotechnology for Biofuels, 9(1), 147-147 (2016)
Jack bean alpha-mannosidase: Amino acid sequencing and N-glycosylation analysis of a valuable glycomics tool.
Kumar G, et al.
Glycobiology, 24(3) (2014)
W Einhoff et al.
Biological chemistry Hoppe-Seyler, 369(3), 165-169 (1988-03-01)
The alpha-mannosidase from Canavalia ensiformis was characterized with respect to molecular mass, glycoprotein nature, amino-acid composition, enzymic properties and action on animal cells. The enzyme is composed of two pairs of subunits (molecular mass 44 and 66 kDa) which form
Collectin-11 promotes the development of renal tubulointerstitial fibrosis
Wu W, et al.
Journal of the American Society of Nephrology, 29, 168-181 (2018)
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Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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Product Information Sheet - M7257

Product Information Sheet

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