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L1882

Lectin from Triticum vulgaris (wheat)

agarose conjugate, saline suspension

Synonym(s):

WGA, Wheat germ agglutinin

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1 mL
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$294.00
5 mL
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$994.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
saline suspension

$294.00


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conjugate

agarose conjugate

Quality Level

form

saline suspension

extent of labeling

5-10 mg per mL

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

capacity

≥0.1 mg/mL binding capacity (ovomucoid)

storage temp.

2-8°C

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This Item
L0636L3892L9640
matrix

cross-linked 4% beaded agarose

matrix

-

matrix

-

matrix

-

form

saline suspension

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

conjugate

agarose conjugate

conjugate

-

conjugate

peroxidase conjugate

conjugate

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

extent of labeling

5-10 mg per mL

extent of labeling

-

extent of labeling

-

extent of labeling

-

General description

Lectins are carbohydrate-binding proteins, found in fungi, plants and animals. The secondary structure of a lectin is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Application

Used for the purification of glycoproteins containing (D-N-acetylglucosamine)2 or N-acetylneuraminic acid.

Biochem/physiol Actions

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Lectins are known to be useful in glycoconjugate characterizing, imaging and targeting. They specifically interact with oligosaccharides, glycoproteins and glycolipids. Hence, lectins are used in microarray assays, enabling efficient glycome profiling. In plants and fungi, lectins defend against pathogens or feeders. They are also involved in host recognition and tissue adhesion.

Physical form

Suspension in 1.0 M NaCl containing 15 mM sodium azide

Preparation Note

Conjugates are prepared from affinity purified lectin.

Storage Class

10 - Combustible liquids

wgk

WGK 3

ppe

Eyeshields, Gloves


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K Paz et al.
The Journal of biological chemistry, 271(12), 6998-7003 (1996-03-22)
A structural analysis has been carried out to determine which part of the intracellular domain of the insulin receptor (IR) beta subunit is involved in direct interaction with the receptor substrates IRS-1 and Shc. Toward this end, the juxtamembrane (JM)
Binding sugars: from natural lectins to synthetic receptors and engineered neolectins
Arnaud J, et al.
Chemical Society Reviews, 42(11), 4798-4813 (2013)
Shohei Wakao et al.
Cellular and molecular life sciences : CMLS, 79(11), 542-542 (2022-10-07)
Stem cells undergo cytokine-driven differentiation, but this process often takes longer than several weeks to complete. A novel mechanism for somatic stem cell differentiation via phagocytosing 'model cells' (apoptotic differentiated cells) was found to require only a short time frame.

Articles

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Protocols

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin.

This page shows how to prepare samples for purification with affinity chromatography.

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Related Content

Glycobiology and Glycoproteomics Brochure

Global Trade Item Number

SKUGTIN
L1882-1ML04061838163929
L1882-5ML04061838163936

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