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I9516

2-Propanol

≥99.5%, for molecular biology, suitable for RNA extraction, DNA extraction, BioReagent

Synonym(s):

sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol

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25 ML

$42.70

4 X 25 ML

$63.40

500 ML

$90.50

1 L

$142.00

4 L

$333.00

$42.70

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About This Item

Linear Formula:
(CH3)2CHOH
CAS Number:
67-63-0
Molecular Weight:
60.10
NACRES:
NA.52
PubChem Substance ID:
24896158
UNSPSC Code:
12352104
EC Number:
200-661-7
MDL number:
MFCD00011674
Beilstein/REAXYS Number:
635639
Assay:
≥99.5%
Grade:
Molecular Biology
Technique(s):
DNA extraction: suitable, RNA extraction: suitable
Bp:
82 °C (lit.)
Vapor pressure:
33 mmHg ( 20 °C), 44 mmHg ( 25 °C)

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Product Name

2-Propanol, BioReagent, ≥99.5%, Molecular Biology

grade

Molecular Biology

Quality Level

200

vapor density

2.1 (vs air)

vapor pressure

33 mmHg ( 20 °C), 44 mmHg ( 25 °C)

product line

BioReagent

assay

≥99.5%

form

liquid

autoignition temp.

750 °F

expl. lim.

2.0-12.7 %, 93 °C

technique(s)

DNA extraction: suitable, RNA extraction: suitable

impurities

≤0.2% water
water

color

colorless

refractive index

n20/D 1.377 (lit.)

bp

82 °C (lit.)

mp

−89.5 °C (lit.)

solubility

water: soluble (completely)

density

0.785 g/mL at 25 °C (lit.)

suitability

suitable for precipitation of nucleic acids

storage temp.

room temp

SMILES string

CC(C)O

InChI

1S/C3H8O/c1-3(2)4/h3-4H,1-2H3

InChI key

KFZMGEQAYNKOFK-UHFFFAOYSA-N

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1 of 4

This Item
593041.010401.13350
2-Propanol BioReagent, ≥99.5%, Molecular Biology

Sigma-Aldrich

I9516

2-Propanol

2-Propanol BioUltra, Molecular Biology, ≥99.5% (GC)

Sigma-Aldrich

59304

2-Propanol

2-Propanol gradient grade for liquid chromatography LiChrosolv®

Supelco

1.01040

2-Propanol

2-Propanol for preparative chromatography Prepsolv™

Supelco

1.13350

2-Propanol

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200

technique(s)

DNA extraction: suitable, RNA extraction: suitable

technique(s)

DNA extraction: suitable, RNA extraction: suitable

technique(s)

HPLC: suitable

technique(s)

-

grade

for molecular biology

grade

for molecular biology

grade

-

grade

-

assay

≥99.5%

assay

≥99.5% (GC)

assay

≥99.9% (GC)

assay

≥99.8% (GC)

solubility

water: soluble (completely)

solubility

water: soluble (completely)

solubility

-

solubility

-

form

liquid

form

liquid

form

liquid

form

liquid

General description

Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.

Protocols for the use of isopropanol:

  • DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.[1]
  • Protein crystallization.[2][3][4]
  • Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.[5][6]

Application

2-Propanol has been used:
  • in DNA and RNA extraction[7][8][9][10]
  • for determination of hydroxyproline levels(The Merck Index, 12th ed)
  • for checking cell viability by MTT assay[11]
  • in microarray hybridization[12]
Suitable for use in DNA precipitation using standard protocols. Also suitable as a solvent for making solutions for molecular biology applications.

pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

target_organs

Central nervous system

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

53.6 °F - closed cup

flash_point_c

12.0 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
0000542038
0000542038
BCCP4716
SHBT5354
SHBT4325

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Find documentation for the products that you have recently purchased in the Document Library.

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Christudas Morais et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 25(5), 1462-1474 (2009-12-29)
Renal cell carcinoma (RCC) is a highly metastatic and lethal disease with few efficacious treatments. Many studies have shown that the ubiquitous transcription factor nuclear factor kappa B (NF-kappaB) plays a key role in the development and progression of many
M J Romão et al.
European journal of biochemistry, 215(3), 729-732 (1993-08-01)
The Desulfovibrio gigas aldehyde oxidoreductase contains molybdenum bound to a pterin cofactor and [2Fe-2S] centers. The enzyme was characterized by SDS/PAGE, gel-filtration and analytical ultracentrifugation experiments. It was crystallized at 4 degrees C, pH 7.2, using isopropanol and MgCl2 as
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, E-E (1989)
S Knapp et al.
Proteins, 23(4), 595-597 (1995-12-01)
The thermostable amylopullulanase from Pyrococcus woesei was crystallized. Crystals, suitable for a crystallographic analysis up to a size of 0.6 mm in their longest dimension, have been obtained by the vapor diffusion method in a solution containing polyethyleneglycol 4000 (PEG
Robert B Nicewonger et al.
Bioorganic & medicinal chemistry letters, 12(14), 1799-1802 (2002-06-28)
In this paper, we describe the synthesis of N-(6-cyano-1,3-dimethyl-2,4-dioxo-5-substituted-1,3-dihydropyridino[2,3-d] pyrimidin-7-yl)imides 1. We will show the synthesis of 1 using both conventional heating and microwave techniques. In addition, the imide was attached to polystyrene and this immobilized imide was equally as
View All Related Papers

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Global Trade Item Number

SKUGTIN
I9516-1L04061841441250
I9516-25ML04061837897221
I9516-4L04061841441267
I9516-4X25ML04061837897238
I9516-500ML04061837897245
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