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H1009

Sigma-Aldrich

Hydrogen peroxide solution

Hydrogen peroxide solution

30 % (w/w) in H2O, contains stabilizer

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$65.60
100 ML
$73.00
500 ML
$188.00

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5 ML
$65.60
100 ML
$73.00
500 ML
$188.00

About This Item

Empirical Formula (Hill Notation):
H2O2
CAS Number:
7722-84-1
Molecular Weight:
34.01
Beilstein/REAXYS Number:
3587191
MDL number:
MFCD00011333
UNSPSC Code:
12352304
PubChem Substance ID:
57654227
NACRES:
NA.77

$65.60

In StockDetails

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vapor pressure

23.3 mmHg ( 30 °C)

Quality Level

200

assay

29.0-32.0% (titration)

form

liquid

shelf life

~2 yr

reaction suitability

reagent type: oxidant

concentration

30 % (w/w) in H2O

technique(s)

cell based assay: suitable
immunohistochemistry: suitable

density

1.110 g/cm3

suitability

suitable for DNA precipitation

application(s)

general analytical
pharmaceutical

storage temp.

2-8°C

SMILES string

OO

InChI

1S/H2O2/c1-2/h1-2H

InChI key

MHAJPDPJQMAIIY-UHFFFAOYSA-N

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General description

Hydrogen peroxide (H2O2), a topical antiseptic, is synthesized in aerobic cells as a byproduct of aerobic respiration. It might also be produced as an output of enzymatic reactions in peroxisomes, mitochondria, or other cell compartments.

Application

Hydrogen peroxide solution has been used:
  • to treat nasal epithelial cells for H2O2 sensitivity assays
  • to treat the human immortal keratinocyte cell line (HaCaT) and the human melanoma cell line (GLL19) to induce senescence
  • to incubate brain sections for bromodeoxyuridine (BrdU)/NeuN staining
  • in cell proliferation assay to incubate neural stem cells (NE-4C) for in vitro experiments

Biochem/physiol Actions

Hydrogen peroxide (H2O2) is mainly used in wound cleaning. It can kill the pathogens through oxidation burst and by producing local oxygen. H2O2 functions as a signaling molecule or second messenger. It plays a key role in gene expression in numerous ways. H2O2 participates in modulating inflammation.

Features and Benefits

This compound is featured on the Phosphoprotein Phosphatases (Tyrosine) page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
For additional information on our range of Biochemicals, please complete this form.

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pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1

Storage Class

5.1B - Oxidizing hazardous materials

wgk_germany

WGK 1

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
MKCX2387
STBL4103
MKCV5253
MKCV9876
SDBB1447

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S V Lennon et al.
Cell proliferation, 24(2), 203-214 (1991-03-01)
Cell death may occur by either of two mechanisms: apoptosis or necrosis. Necrosis, the first type of cell death to be recognized, is an uncontrolled degenerative phenomenon invariably caused by noxious stimuli and is the result of irreversible failure of
Akitoshi Nakashima et al.
Scientific reports, 9(1), 11466-11466 (2019-08-09)
Pregnancy is a stress factor culminating into mild endoplasmic reticulum (ER) stress, which is necessary for placental development. However, excessive or chronic ER stress in pre-eclamptic placentas leads to placental dysfunction. The precise mechanisms through which excessive ER stress impacts
Shoichi Iriguchi et al.
Blood, 125(2), 370-382 (2014-10-29)
Although overexpression of T-bet, a master transcription factor in type-1 helper T lymphocytes, has been reported in several hematologic and immune diseases, its role in their pathogenesis is not fully understood. In the present study, we used transgenic model mice
Osamu Sakai et al.
Investigative ophthalmology & visual science, 56(1), 538-543 (2015-01-13)
The purpose of the present study was to investigate the role of glutathione peroxidase 4 (GPx4) in conjunctival epithelial cells. An immortalized human conjunctival epithelial cell line was used. Cells were transfected with catalase, GPx1, GPx4, SOD1, SOD2, or control
Yan Zhu et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(24), 9211-9224 (2015-06-19)
Radial glial cells are the neural progenitors of the developing CNS and have long radial processes that guide radially migrating neurons. The integrity of the radial glial scaffold, in particular proper adhesion between the endfeet of radial processes and the
View All Related Papers

Articles

Colorimetric Detection of Western blot using 4-Chloro-1-Naphthol Solution Protocol

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

Protocols

Enzymatic Assay of Catalase (EC 1.11.1.6)

This procedure may be used for all Catalase products.

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

Enzymatic Assay of Peroxidase (EC 1.11.1.7)

This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.

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Questions

1–10 of 19 Questions  

  1. How much we need to add 30% H2O2 in 1mL of solution(water as solvent) to make100mM molarity?

    1 answer

    1. The molarity of this product is calculated to be 9.791 M based on a density of 1.11 g/mL, a formula weight of 34.01 g/mol, and a concentration of 30 w/w%. Here is the calculation:
      Molarity = Density x concentration x 1000 / formula weight = 1.11 x 0.3 x 1000 / 34.01 = 9.791 M.

      To make a 100 mM solution, use the Dilution Formula: C1V1 = C2V2 to find the volume V1 of the 30% H2O2 solution needed.
      C1 = 9.791 M (concentration of the stock solution)
      C2 = 0.1 M (desired concentration)
      V2 = 0.001 L + V1 (final volume, which is the sum of 1 mL of water and the volume of H2O2 added)

      Rearrange the formula to solve for V1:
      9.791 x V1 = 0.1 x (0.001 + V1)
      V1 = 0.1 x 0.001 / (9.791 - 0.1) = ~0.0000103 L = ~10.3 µL

      Therefore, approximately 10.3 µL of this product is required for a 100 mM H2O2 solution when mixed with 1 mL of water.

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  4. What stabilizer was added? I can't find any information in the DSD or spec sheet.

    1 answer

    1. This product contains Dipicolinic acid at 5 – 10ppm as a stabilizer.

      Helpful?

  5. how can we determinate H2O2 concentration in case of 30% by titration , i found way to do this with sodium thiosulfate but they mentioned that is for 0.1% - 5 % con. - can i use it ?

    1 answer

    1. Using a procedure meant for lower concentrations of hydrogen peroxide may lead to less accurate results. Solvay chemicals has a method for determination of the concentration of hydrogen peroxide (20-70%) by titration with potassium permanganate solution: https://www.solvay.com/sites/g/files/srpend221/files/2019-10/H2O2%20Determination%20of%20Hydrogen%20Peroxide%20Concentration%20-20%20to%2070-.pdf

      Helpful?

  6. - CONTACT TIME 문의 해당 과산화수소 수용액 30% 이용하여 소독 진행 시 검증되어 있는 컨택타임이 있는지 문의 드립니다.

    1 answer

    1. Unfortunately, there is no verified contact time for the use of product H1009 in disinfecting.

      Helpful?

  7. Hi, I would like to get information on this product (H2O2 Cat # H1009-5ML) for Lot numbers MKCL6061 and MKCJ1034 to know their expiration dates. Please help me get this information. Thanks.

    1 answer

    1. This product is not assigned an expiration date. It is assigned a recommended retest date of approximately one year after the quality release date. A recommended retest date is the time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

      Please see the link below to access a specific lot or to review a sample Certificate:
      https://www.sigmaaldrich.com/product/sigma/H1009#product-documentation

      Helpful?

  8. how much we need to add 30% H2O2 in 7ml of solution(water as solvent) to make 5mM molarity....could you please tell with calculation?

    1 answer

    1. The molarity of this product will change slightly from lot to lot, based on the final concentration. The concentration range is 29 - 32 %. The molarity can be calculated using the basic equation (density in g/L x final percentage)/molecular weight. The molarity of a 30 % solution of this product can be calculated by the following:
      (1110 g/L X 0.30) / 34.01 = 9.79 M.

      The volume of product required to prepare 7 mL of 0.005 M H2O2 would be 3.6 ul. For ease of pipetting, it may be more practical to prepare a larger volume. For example:
      Use 3.6 uL to prepare 7 mL at 5 mM
      Use 7.7 uL to prepare 15 mL at 5 mM
      Use 10.3 uL to prepare 20 mL at 5 mM

      Alternatively, an intermediate working solution can be prepared that would require further dilution.
      To prepare a 1 M stock from the 9.79 M concentrate, a dilution may be prepared using the M1V1 =M2V2 formula.
      In this case, 1.02 mL of the 9.79 M stock is diluted to 10 mL to achieve a 1 M concentration.
      From the 1 M solution dilute 0.035 mL (35 ul) to a final volume of 7 mL to achieve a 5 mM concentration.

      Helpful?

  9. 30% is w/w ratio or its w/v ratio and can you give formula to calculate molarity of this H2O2 30% solution?

    1 answer

    1. As stated on the product detail page, this is hydrogen peroxide 30% w/w in H2O.

      The molarity of this product will change slightly from lot to lot, based on the final concentration. The concentration range is 29 - 32 %. The molarity can be calculated using the basic equation (density in g/L x final percentage)/molecular weight. The molarity of a 30% solution of this product can be calculated by the following:
      (1197 g/L X 0.30) / 34.01 = 9.79 M

      Helpful?

  10. How do I check the concentration of H2O2 for a solution that has been stored at 4 degrees Celsius for about a year? Do I check the absorbance at 240nm?

    1 answer

    1. The product concentration is 30 % (w/w) in H2O. This product has a shelf life of approximately 2 years. The concentration of this product is determined by iodometric titration. See the link below to review an application note provided by Mettler Toledo: https://www.mt.com/us/en/home/supportive_content/ana_chem_applications/titration/M860.html

      To further discuss further, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.

      Helpful?

1–10 of 19 Questions  

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