The minimum activity of this ?-glucanase is 1 unit per milligram. The activity varies from lot to lot and is reported in the Certificate of Analysis. This product has not been tested for any additional protease activity. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/g4423#product-documentation
G4423
β-Glucanase from Trichoderma longibrachiatum
Synonym(s):
endo-1,3(4)-β-glucanase, endoglucanase
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100 G
$228.00
$228.00
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About This Item
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form
powder
specific activity
≥1.0 units/mg solid
composition
protein, ≥10%
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
, Enabling
Quality Level
1 of 4
This Item | G0251 | X3876 | C6137 |
|---|---|---|---|
| specific activity ≥1.0 units/mg solid | specific activity ≥1,000,000 units/g solid | specific activity 100-300 units/mg protein | specific activity ≥200 units/g solid |
| form powder | form solid | form lyophilized powder | form lyophilized powder (essentially salt free) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| composition protein, ≥10% | composition - | composition Protein, ~50% biuret | composition - |
| greener alternative product characteristics Waste Prevention | greener alternative product characteristics - | greener alternative product characteristics Waste Prevention | greener alternative product characteristics - |
| sustainability Greener Alternative Product | sustainability - | sustainability Greener Alternative Product | sustainability - |
Application
β -Glucanase was used as a cellulase enzyme in the combined biological and chemical pretreatment method for lignocellulosic ethanol production from energy cane. It was also used in the enzymatic saccharification of cellulose and production of ethanol. [1]
Biochem/physiol Actions
General description
β -Glucanase represents a group of carbohydrate enzymes which break down glycosidic bonds within beta-glucan.[4] It forms the main constituent of fungal cell walls and could be a potential structural and storage polysaccharide of marine macro-algae. It has the ability to degrade fungal cell walls and may be involved in defense mechanism of plants against pathogenic fungi.[4]
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles.
Other Notes
One unit will liberate 1.0 μmole of glucose from cellulose in one hr at pH 5.0 at 37 °C (2 hr incubation time).
Physical form
contains maltodextrin, silica and sodium benzoate
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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David J Adams
Microbiology (Reading, England), 150(Pt 7), 2029-2035 (2004-07-17)
The fungal cell wall is a complex structure composed of chitin, glucans and other polymers, and there is evidence of extensive cross-linking between these components. The wall structure is highly dynamic, changing constantly during cell division, growth and morphogenesis. Hydrolytic
Ravindra Bansal et al.
Current genetics, 65(4), 953-964 (2019-03-07)
Trichoderma spp. are widely used as commercial biofungicides, and most commercial formulations are conidia based. Identification of genes that regulate conidiation would thus be of help in genetic reprogramming of these species to optimize sporulation. In this study, we constructed
Marie-Therese Eckstein et al.
Current biology : CB, 30(23), 4799-4807 (2020-10-10)
The human gut microbiota is composed of diverse microbes that not only compete but also rely on one another for resources and access to microhabitats in the intestine [1, 2]. Indeed, recent efforts to map the microbial biogeography of the
Combined biological and chemical pretreatment method for lignocellulosic ethanol production from energy cane;
Sara Shields, Raj Boopathy
Renewable Bioresources null
Nhuan P Nghiem et al.
Applied biochemistry and biotechnology, 165(3-4), 870-882 (2011-06-15)
A fermentation process, which was designated the enhanced dry grind enzymatic (EDGE) process, has recently been developed for barley ethanol production. In the EDGE process, in addition to the enzymes normally required for starch hydrolysis, commercial β-glucanases were used to
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How much protease activity is present?
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Is this product suitable for use in food preparation?
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No. Unless otherwise stated, this product is intended for research use only. It is not suitable for any other purpose, which includes, but is not limited to, unauthorized commercial use, in vitro diagnostic use, or therapeutic use in humans or animals.
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Hi, I am planning to use β-Glucanase G4423 to digest the Pyhtium inosidieus cell wall to let the probes for In situ hybridozation . May I know the conditions, concentration and dilution buffer for this product? Thanks Best
1 answer-
This product has not been testd in In Situ Hybridization in house. Below is a link to an article citing this product in ISH.
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