The minimum activity of this ?-glucanase is 1 unit per milligram. The activity varies from lot to lot and is reported in the Certificate of Analysis. This product has not been tested for any additional protease activity. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/g4423#product-documentation
G4423
β-Glucanase from Trichoderma longibrachiatum
Synonym(s):
endo-1,3(4)-β-glucanase, endoglucanase
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100 G
$213.00
$213.00
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100 G
$213.00
About This Item
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
$213.00
Please contact Customer Service for Availability
form
powder
Quality Level
specific activity
≥1.0 units/mg solid
composition
protein, ≥10%
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
, Enabling
General description
β -Glucanase represents a group of carbohydrate enzymes which break down glycosidic bonds within beta-glucan.[1] It forms the main constituent of fungal cell walls and could be a potential structural and storage polysaccharide of marine macro-algae. It has the ability to degrade fungal cell walls and may be involved in defense mechanism of plants against pathogenic fungi.[1]
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles.
Application
β -Glucanase was used as a cellulase enzyme in the combined biological and chemical pretreatment method for lignocellulosic ethanol production from energy cane. It was also used in the enzymatic saccharification of cellulose and production of ethanol. [2]
Biochem/physiol Actions
Unit Definition
One unit will liberate 1.0 μmole of glucose from cellulose in one hr at pH 5.0 at 37 °C (2 hr incubation time).
Physical form
contains maltodextrin, silica and sodium benzoate
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Certificates of Analysis (COA)
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How much protease activity is present?
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Is this product suitable for use in food preparation?
1 answer-
No. Unless otherwise stated, this product is intended for research use only. It is not suitable for any other purpose, which includes, but is not limited to, unauthorized commercial use, in vitro diagnostic use, or therapeutic use in humans or animals.
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Hi, I am planning to use β-Glucanase G4423 to digest the Pyhtium inosidieus cell wall to let the probes for In situ hybridozation . May I know the conditions, concentration and dilution buffer for this product? Thanks Best
1 answer-
This product has not been testd in In Situ Hybridization in house. Below is a link to an article citing this product in ISH.
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