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F7252

Sigma-Aldrich

Fast Green FCF

Dye content ≥85 %, powder

Synonym(s):

Food green 3

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About This Item

Linear Formula:
C37H34N2O10S3Na2
CAS Number:
2353-45-9
Molecular Weight:
808.85
Colour Index Number:
42053
Beilstein/REAXYS Number:
5718212
EC Number:
219-091-5
MDL number:
MFCD00013053
UNSPSC Code:
12352204
PubChem Substance ID:
57654190
NACRES:
NA.83

product name

Fast Green FCF, Dye content ≥85 %

form

powder

composition

Dye content, ≥85%

mp

290 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL, clear

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O

InChI

1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2

InChI key

RZSYLLSAWYUBPE-UHFFFAOYSA-L

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General description

Fast Green FCF is one of the triarylmethane dyes, that is biocompatible. This triphenylmethane color additive is mainly used in coloring food, drugs and cosmetics.

Application

Fast Green FCF has been used for in staining of menisci from rabbit and of osteochondral tissues.
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Biochem/physiol Actions

Fast Green FCF can be considered as a safe stain to detect tumor. In recent years, this has gained importance as an important drug to treat amyloid-deposit diseases.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

hcodes

H335,H341

Hazard Classifications

Muta. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

352.4 °F - closed cup

flash_point_c

178 °C - closed cup

ppe

Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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