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EDEGLY

Enzymatic Protein Deglycosylation Kit

Synonym(s):

Glycosylation Release Kit

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1 kit
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$1,300.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202

$1,300.00

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Quality Level

200

conjugate

(N-linked), (O-linked)

shipped in

wet ice

storage temp.

2-8°C

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This Item
PP0510CS0008NDEGLY
Enzymatic Protein Deglycosylation Kit

Sigma-Aldrich

EDEGLY

Enzymatic Protein Deglycosylation Kit

GlycoProfile™ IV Chemical Deglycosylation Kit TFMS Deglycosylation System

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PP0510

GlycoProfile™ IV Chemical Deglycosylation Kit

Enzymatic Protein Biotinylation Kit for biotinylation of up to 20 mg target protein

Sigma-Aldrich

CS0008

Enzymatic Protein Biotinylation Kit

Native Protein Deglycosylation Kit

Sigma-Aldrich

NDEGLY

Native Protein Deglycosylation Kit

conjugate

(N-linked)

conjugate

-

conjugate

-

conjugate

(N-linked)

shipped in

wet ice

shipped in

-

shipped in

dry ice

shipped in

wet ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−70°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Enzymatic protein deglycosylation kit contains all the enzymes and reagents required for the complete removal of N-linked and simple O-linked carbohydrates from glycoproteins. This kit is employed to cleave the complex Core 2 O-linked carbohydrates including those containing polylactosamine.

Application

Enzymatic protein deglycosylation kit has been used in:
  • protein deglycosylation[1]
  • deglycosylation of tissue factor pathway inhibitor (TFPI) α and β[2]
  • deglycosylation of native exo-β-(1,3)-glucanase EXG1[3]

Enzymatic Protein Deglycosylation Kit has been used for the deglycosylation of various enzymes.

Features and Benefits

Enzymatic protein deglycosylation kit has the following features:
  • two or more mg of glycoprotein is deglycosylated
  • single reaction at neutral pH
  • native & denaturing procedures
  • no degradation of protein
  • O-linked sugars containing polysialic acid are eliminated
  • control glycoprotein provided

Kit Components Only

Product No.
Description
  • PNGase F 1 mL/vial
  • O-Glycosidase 20 μL
  • α-2(3,6,8,9)-Neuraminidase 20 μL
  • Fetuin Control .5 mg
  • 5× Reaction Buffer .2 mL
  • Denaturation Solution .1 mL
  • Triton™ X-100 .1 mL
  • β-1→4-Galactosidase, positionally specific 20 μL
  • β-N-Acetylglucosaminidase 20 μL
See All (9)

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Product No.
Description
Pricing

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
0000377170
0000377170
SLCM3146
SLCN4952
SLCK4329

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Guillain-Barre syndrome: First description of a snake envenomation aetiology
Neil J, et al.
Journal of Neuroimmunology, 242(1-2), 72-77 (2012)
Julien Chapuis et al.
Human molecular genetics, 20(10), 2026-2036 (2011-03-02)
Alzheimer's disease (AD), the most common neurodegenerative disorder, is characterized by cerebral deposition of amyloid-β (Aβ), a series of peptides derived from the processing of the amyloid-β precursor protein (APP). To identify new candidate genes for AD, we recently performed
Angela Mika et al.
PLoS neglected tropical diseases, 5(9), e1331-e1331 (2011-10-08)
Pruritic scabies lesions caused by Sarcoptes scabiei burrowing in the stratum corneum of human skin facilitate opportunistic bacterial infections. Emerging resistance to current therapeutics emphasizes the need to identify novel targets for protective intervention. We have characterized several protein families
Primavera Alvarado et al.
Journal of microbiological methods, 175, 105981-105981 (2020-06-15)
The diagnosis of histoplasmosis depends on various approaches: direct clinical examination, fungus isolation from cultures of clinical samples, histopathological evaluation, and serological testing. In serodiagnostic assays, the Histoplasma capsulatum H and M antigenic glycoproteins have been extensively used. However, both
Axel T Lehrer et al.
Vaccine, 36(22), 3090-3100 (2017-02-22)
Infections with filoviruses in humans are highly virulent, causing hemorrhagic fevers which result in up to 90% mortality. In addition to natural infections, the ability to use these viruses as bioterrorist weapons is of significant concern. Currently, there are no
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Pretreatment with Mucinase StcE increases glycopeptide identification from mucin samples, enhancing sample preparation efficiency for glycopeptide analysis.

Glycoprotein Deglycosylation

Information about Glycoprotein deglycosylation. The diversity of oligosaccharide structures, both O-linked and N-linked, often results in heterogeneity in the mass and charge of glycoproteins.

Strategies for Deglycosylating N-Linked Glycans

Explore strategies for releasing N-linked glycans with PNGase F, PNGase A & native & sequential deglycosylation with endoglycosidases & exoglycosidases.

Post Translational Modification

Protein modifications are crucial for disease study. Analysis methods are key.

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Global Trade Item Number

SKUGTIN
EDEGLY-1KT04061833609392

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