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Endoglycosidase F1

Name: Endoglycosidase F1
Brand: SIGMA

Cleaves within N-linked glycans, suitable for Mass Spectrometry, from Elizabethkingia miricola, recombinant, expressed in E. coli

Synonym(s):

Endo F1, Endo-β-N-acetylglucosaminidase F1, Endoglycosidase F1 from Chryseobacterium meningosepticum, Endoglycosidase F1 from Elizabethkingia meningoseptica, Endoglycosidase F1 from Flavobacterium meningosepticum

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

3.2.1.96 (BRENDA, IUBMB)

MDL number:

MFCD00146843

Specific activity:

≥16 U/mg

Recombinant:

expressed in E. coli

Pricing and availability is not currently available.

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Product Name

Endoglycosidase F1 from Elizabethkingia miricola, recombinant, expressed in E. coli, ≥16 U/mg, buffered aqueous solution

recombinant

expressed in E. coli

Quality Level

200

conjugate

(N-linked)

form

buffered aqueous solution

specific activity

≥16 U/mg

mol wt

32 kDa

shipped in

wet ice

storage temp.

2-8°C

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Show Differences

1 of 1

This Item	E0639	E2264	324725
Sigma-Aldrich E9762 Endoglycosidase F1 from Elizabethkingia miricola	Sigma-Aldrich E0639 Endoglycosidase F2 from Elizabethkingia miricola	Sigma-Aldrich E2264 Endoglycosidase F3 from Elizabethkingia miricola	Sigma-Aldrich 324725 Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli
specific activity ≥16 U/mg	specific activity 20 U/mg	specific activity 30 U/mg	specific activity ≥16 units/mg protein, ≥17 units/mL
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli
form buffered aqueous solution	form solution	form solution	form liquid
mol wt 32 kDa	mol wt 32 kDa	mol wt 32 kDa	mol wt -
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

General description

Endoglycosidase F1 from Elizabethkingia miricola is a glycan specific enzyme.

Application

Cleaves asparagine-linked or free oligomannose and hybrid, but not complex, oligosaccharides.

Endoglycosidase F1 from Elizabethkingia miricola has been used to remove binding of human hemochromatosis protein (HFE) to cation independent mannose-6-phosphate receptor (CI-MPR) and to glycosylate fucosylated N-glycopeptides.

Biochem/physiol Actions

Endoglycosidase F1 from Elizabethkingia miricola plays an important role in cleavage of glycan structures from the protein by cleaving between the two N-acetylglucosamine residues of the chitobiose core. It mediates high mannose and hybrid oligosaccharides cleavage.

Packaging

Supplied with 5× Reaction Buffer, 250 mM NaH₂PO₄, pH 5.5.

Physical form

Aseptically filled solution in 20 mM Tris-HCl pH 7.5

Other Notes

One unit will release N-linked oligosaccharides from 1 μmole of denatured Ribonuclease B in 1 minute at 37 °C, pH 5.5.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000502927

0000487796

0000492661

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EndoS2 is a unique and conserved enzyme of serotype M49 group A Streptococcus that hydrolyses N-linked glycans on IgG and alpha-acid glycoprotein

Jonathan S, et al.

BioChemistry: An Indian Journal, 455, 107-118 (2013)

Receptor activity-modifying protein 1 determines the species selectivity of non-peptide CGRP receptor antagonists

Mallee JJ, et al.

The Journal of Biological Chemistry, 277, 14294-14298 (2002)

In vitro binding of HFE to the cation-independent mannose-6 phosphate receptor

Schimanski LM, et al.

Blood Cells, Molecules and Diseases, 43(2), 180-193 (2009)

Quantitative analysis of core fucosylation of serum proteins in liver diseases by LC-MS-MRM

Ma J, et al.

Journal of proteomics, 189, 67-74 (2018)

[Heterologous interferons synthesis in yeast Pichia pastoris].

M V Padkina et al.

Prikladnaia biokhimiia i mikrobiologiia, 46(4), 448-455 (2010-09-29)

The HuIFNA16, HuIFNB, and BoIFNG genes encoding human [alpha]16, beta-interferons and bovine gamma-interferon were cloned under the control of the yeast Pichia pastoris AOX1 gene promoter. The yeast strains producing heterologous interferons intracellularly and extracellularly were constructed. There was no

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Global Trade Item Number

SKU	GTIN
E9762-1UN	04061833609200
E9762-0.3UN	04061832856445

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