This product contains HEPES buffer salts and a stabilizing carbohydrate component. The material may be reconstituted in water at 1mg/mL. Solution stability studies have not been performed, however, the stock solution is expected to be stable for 1 - 2 months when aliquoted and stored at −20°C. Avoid repeated freeze/thaw cycles. A datasheet is not available for this product. Please see the link below to review the enzyme assay protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/198/734/c9584enz.pdf
C9584
Carboxypeptidase B from porcine pancreas
lyophilized powder
Synonym(s):
Peptidyl-L-lysine(L-arginine) hydrolase, Protaminase
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Product Name
Carboxypeptidase B from porcine pancreas, lyophilized powder
SMILES string
S(Cc3ccccc3)C(=O)C(NC(=O)C2N(CCC2)C(=O)C(NC(=O)C(NC(=O)CCC(=O)O)C)C)Cc1ccccc1
InChI key
TWURVFFNODFJBJ-UHFFFAOYSA-N
InChI
1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
biological source
Porcine pancreas
grade
Proteomics Grade
form
lyophilized powder
specific activity
≥125 units/mg protein
mol wt
34,000 Da± 600
purified by
affinity chromatography
composition
Protein, 40-70%
foreign activity
carboxypeptidase A ≤1%
chymotrypsin ≤0.1%
trypsin ≤5%
storage temp.
−20°C
Quality Level
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Related Categories
1 of 4
This Item | C9268 | C9658 | 217356 |
|---|---|---|---|
| grade Proteomics Grade | grade Proteomics Grade | grade - | grade - |
| specific activity ≥125 units/mg protein | specific activity ≥50 units/mg protein | specific activity ≥3 units/mg protein | specific activity ≥100 units/mg protein |
| biological source Porcine pancreas | biological source - | biological source - | biological source Porcine pancreas |
| form lyophilized powder | form ready-to-use solution | form lyophilized powder | form lyophilized |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 34,000 Da± 600 | mol wt ~35 kDa | mol wt homodimer ~90 kDa | mol wt - |
Analysis Note
Protein determined by biuret.
Application
Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis.
The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format.[1] It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.[2]
Biochem/physiol Actions
Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2′-dipyridyl.[3]
Other Notes
One unit will hydrolyze 1.0 μmole of hippuryl-L-arginine per min at pH 7.65 at 25 °C.
Packaging
Package size based on protein content
Physical form
Contains HEPES buffer salts, zinc chloride and carbohydrate
Preparation Note
Treated with protease inhibitor, AEBSF, to eliminate serine protease activity.
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Andrew Lee et al.
Langmuir : the ACS journal of surfaces and colloids, 27(18), 11560-11574 (2011-08-13)
This article describes the use of capillary electrophoresis (CE) to examine the influence of different cations (C(+); C(+) = Na(+) and tetra-n-alkylammonium, NR(4)(+), where R = Me, Et, Pr, and Bu) on the rates of denaturation of bovine carbonic anhydrase
E Curry et al.
Theriogenology, 78(2), 308-314 (2012-04-28)
Currently, there is no method of accurately and non-invasively diagnosing pregnancy in polar bears. Specific proteins may exhibit altered profiles in the feces of pregnant bears, but predicting appropriate candidate proteins to investigate is speculative at best. The objective of
Marc Ferrer et al.
Analytical biochemistry, 317(1), 94-98 (2003-05-06)
An epitope-unmasking, homogeneous time-resolved fluorescence (HTRF) assay has been developed for measuring carboxypeptidase B (CPB) activity in a miniaturized high-throughput screening format. The enzyme substrate (biotin-RYRGLMVGGVVR-OH) is cleaved by CPB at the C terminus, causing release of the C-terminal Arg
Marco Candela et al.
Journal of bacteriology, 189(16), 5929-5936 (2007-06-15)
Bifidobacteria constitute up to 3% of the total microbiota and represent one of the most important health-promoting bacterial groups of the human intestinal microflora. The presence of Bifidobacterium in the human gastrointestinal tract has been directly related to several health-promoting
Laboratory diagnosis of acute pancreatitis: in search of the Holy Grail.
Lippi, G. et al.
Clinical Laboratory Science : Journal of the American Society For Medical Technology, 49, 18-31 (2012)
Related Content
QC Methods
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Chciałam zapytać jak przygotować roztwór Carboxypeptidase B from porcine, który występuje w postaci liofilizowanego proszku? Jest dostępna instrukcja?
1 answer-
Helpful?
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Can Product C9584, Carboxypeptidase B from porcine pancreas be used to remove basic C-terminal residues of tryptic peptides?
1 answer-
The use of Product C9584, Carboxypeptidase B from porcine pancreas to remove basic C-terminal residues of tryptic peptides has been described in the literature. Please see the following as an example:The Protein Protocols Handbook, J.M. Walker, Ed., pp. 569, Humana Press, Totawa NJ (1996)
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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