This product contains HEPES buffer salts and a stabilizing carbohydrate component. The material may be reconstituted in water at 1mg/mL. Solution stability studies have not been performed, however, the stock solution is expected to be stable for 1 - 2 months when aliquoted and stored at −20°C. Avoid repeated freeze/thaw cycles. A datasheet is not available for this product. Please see the link below to review the enzyme assay protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/198/734/c9584enz.pdf
C9584
Carboxypeptidase B from porcine pancreas
lyophilized powder
Synonym(s):
Peptidyl-L-lysine(L-arginine) hydrolase, Protaminase
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1 MG
$111.00
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$384.00
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1 MG
$111.00
5 MG
$384.00
About This Item
$111.00
Please contact Customer Service for Availability
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biological source
Porcine pancreas
Quality Level
grade
Proteomics Grade
form
lyophilized powder
specific activity
≥125 units/mg protein
mol wt
34,000 Da± 600
purified by
affinity chromatography
composition
Protein, 40-70%
foreign activity
carboxypeptidase A ≤1%
chymotrypsin ≤0.1%
trypsin ≤5%
storage temp.
−20°C
SMILES string
S(Cc3ccccc3)C(=O)C(NC(=O)C2N(CCC2)C(=O)C(NC(=O)C(NC(=O)CCC(=O)O)C)C)Cc1ccccc1
InChI
1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
InChI key
TWURVFFNODFJBJ-UHFFFAOYSA-N
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Application
Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis.
The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format.[1] It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.[2]
Biochem/physiol Actions
Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2′-dipyridyl.[3]
Packaging
Package size based on protein content
Unit Definition
One unit will hydrolyze 1.0 μmole of hippuryl-L-arginine per min at pH 7.65 at 25 °C.
Physical form
Contains HEPES buffer salts, zinc chloride and carbohydrate
Preparation Note
Treated with protease inhibitor, AEBSF, to eliminate serine protease activity.
Analysis Note
Protein determined by biuret.
inhibitor
Product No.
Description
Pricing
substrate
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Customers Also Viewed
Marc Ferrer et al.
Analytical biochemistry, 317(1), 94-98 (2003-05-06)
An epitope-unmasking, homogeneous time-resolved fluorescence (HTRF) assay has been developed for measuring carboxypeptidase B (CPB) activity in a miniaturized high-throughput screening format. The enzyme substrate (biotin-RYRGLMVGGVVR-OH) is cleaved by CPB at the C terminus, causing release of the C-terminal Arg
E Curry et al.
Theriogenology, 78(2), 308-314 (2012-04-28)
Currently, there is no method of accurately and non-invasively diagnosing pregnancy in polar bears. Specific proteins may exhibit altered profiles in the feces of pregnant bears, but predicting appropriate candidate proteins to investigate is speculative at best. The objective of
Andrew Lee et al.
Langmuir : the ACS journal of surfaces and colloids, 27(18), 11560-11574 (2011-08-13)
This article describes the use of capillary electrophoresis (CE) to examine the influence of different cations (C(+); C(+) = Na(+) and tetra-n-alkylammonium, NR(4)(+), where R = Me, Et, Pr, and Bu) on the rates of denaturation of bovine carbonic anhydrase
Lawrence L K Leung et al.
Advances in experimental medicine and biology, 632, 61-69 (2008-11-26)
Thrombin-activatable procarboxypeptidase B (proCPB or thrombin-activatable fibrinolysis inhibitor or TAFI) is a plasma procarboxypeptidase that is activated by the thrombin-thrombomodulin complex on the vascular endothelial surface. The activated CPB removes the newly exposed carboxyl terminal lysines in the partially digested
Saurabh Chatterjee et al.
Free radical biology & medicine, 46(4), 454-461 (2008-12-04)
Post-translational modification of proteins due to exposure to radicals and other reactive species are markers of metabolic and inflammatory oxidative stress such as sepsis. This study uses the nitrone spin-trap DMPO and a combination of immuno-spin trapping and mass spectrometry
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Chciałam zapytać jak przygotować roztwór Carboxypeptidase B from porcine, który występuje w postaci liofilizowanego proszku? Jest dostępna instrukcja?
1 answer-
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Can Product C9584, Carboxypeptidase B from porcine pancreas be used to remove basic C-terminal residues of tryptic peptides?
1 answer-
The use of Product C9584, Carboxypeptidase B from porcine pancreas to remove basic C-terminal residues of tryptic peptides has been described in the literature. Please see the following as an example:The Protein Protocols Handbook, J.M. Walker, Ed., pp. 569, Humana Press, Totawa NJ (1996)
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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